慢性乙肝患者外周血单个核细胞凋亡及细胞因子检测的意义

张飚; 隋涛; 于秀英; 曹永献; 姚远; 何宏

慢性乙肝患者外周血单个核细胞凋亡及细胞因子检测的意义

详细信息

中图分类号: R512.62
计量
- 文章访问数: 2104
- HTML全文浏览量: 20
- PDF下载量: 719
- 被引次数: 0
出版历程
- 出版日期: 2004-03-20

Significance of detecting apoptosis of PBMC and cytokine of supernatant induced by PHA in chronic hepatitis B

摘要

摘要: 探讨外周血单个核细胞凋亡和Th1/Th2型细胞因子表达在慢性乙肝发病中的作用。采用PI染色法检测单个核细胞凋亡 ,ELISA法检测IL - 2、IFN -γ、IL - 4、IL - 10等细胞因子水平。慢性乙肝患者单个核细胞凋亡明显高于对照组（P <0 0 1） ,随着抗原刺激时间的延长细胞凋亡增加 ,有显著性意义（P <0 0 1）。IL - 2、IFN -γ水平低于正常对照组（P <0 0 1） ,其浓度随着培养时间的延长有轻度减低 ,但无显著意义（P >0 0 5 ）。IL - 4、IL - 10水平高于正常对照组（P <0 0 1） ,其浓度随着培养时间的延长逐渐升高并且差异有显著意义（P <0 0 1）。慢性乙肝患者外周血淋巴细胞凋亡增强及Th1/Th2免疫调节紊乱 ,使细胞免疫功能减弱 ,淋巴细胞凋亡和Th1/Th2的失衡在慢性乙肝发病中起一定的作用。
- 慢性乙肝 /
- 细胞凋亡 /
- 细胞因子
Abstract: To investigate the significance of PBMC apoptosis and Th1/Th2 cytokine in the pathogenesis of chronic hepatitis B The apoptosis was measured by propidium iodide staining and the IL-2、IFN-γ、IL-4、IL-10 of PBMC induced by PHA was detected with the ELISA The apoptosis of PBMC was higher in hepatitis B than in control group (P<0.01) . There were significant of the increasing apoptosis in hepatitis B when the cultured time was lengthened (P<0.01) . The level of IL-2, IFN-γ was lower in hepatitis B than that in control group (P<0.01) , with the cultured time lengthening, the level of IL-2, IFN-γ slightly decreased while there were no significant (P>0.05) .The level of IL-4、IL-10 was higher in hepatitis B than that in control group (P<0.01) . And the level became higher with the cultured time lengthening, in addition there was significant (P<0.01) .The cell-mediated immunity was weakened because of the strengthened apoptosis of PBMC and imbalance of Th1/Th2 in chronic hepatitis B, lymphocyte apoptosis and imbalance of Th1/Th2 have somewhat function in the pathogenesis of chronic hepatitis B.
- chronic hepatitis B /
- cell apoptosis /
- cytokine

HTML全文

参考文献(10)

[1] MaherS , ToomeyD , CondronC , etal.Activation-inducedcelldeath:thecontroversialroleofFasandFasligandinimmuneprivilegeandtumourcounterattack[J].ImmunolCellBiol, 2002, 80 (2) ∶131-7.

[2] NakamuraK , YuhK , SugyoS , etal.ApoptosisobservedinperipheralTlymphocytesfrompatientswithchronichepatitisB[J].Gastroenterology.1996, 111 (1) ∶156-64.

[3] AbbasAK , MurphyKM , SherA , etal.FunctionaldiversityofhelperTlymphocytes[J]Nature, 1996, 383 (6603) ∶787-93.

[4] DelPreteG , MaggiE , RomagnaniS , etal.HumanTh1andTh2cells:functionalproperties, mechanismsofregulation, androleindisease[J].LabInvest, 1994, 70 (3) ∶299-306.

[5] HalickaDH , PozarowskiP , ItaM , etal.Enhancementofactivation-inducedapoptosisoflymphocytesbythecytotoxicribonucleaseonconase (Ranpirnase) [J].IntJOncol, 2002, 21 (6) ∶1245-50.

[6] FukudaR , IshimuraN , NguyenTX , etal.TheexpressionofIL -2, IL-4andinterferon-gamma (IFN -gamma) mRNA/usingliverbiopsiesatdifferentphasesofacuteexacerbationofchronichepatitisB[J].ClinExpImmunol, 1995, 100 (3) ∶446-51.

[7] HsuHY , ChangMH , NiYH , etal.CytokinereleaseofperipheralbloodmononuclearcellsinchildrenwithchronichepatitisBvirusinfection[J].JPediatrGastroenterolNutr, 1999, 29 (5) ∶540-5.

[8] PriimiagiLS , TefanovaVT , TalloTG , etal.〔Th1-cytokinesinchronichepatitisBandC〕[J].VoprVirusol, 2002, 47 (3) ∶23-7.

[9]邢同京, 章廉, 骆抗先, 等.Th1/Th2类细胞因子对慢性乙型肝炎患者淋巴细胞凋亡的影响[J].解放军医学杂志, 2000, 25 (1) ∶16-18.

[10] SchlaakJF , TullyG , LohrHF , etal.ThepresenceofhighamountsofHBV -DNAinserumisassociatedwithsuppressedcostimulatoryeffectsofinterleukin-12onHBV -inducedimmuneresponse[J].Hepatol, 1999, 30 (3) ∶353-8.

施引文献

资源附件(0)

访问统计