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Relationship Between M30 Expression and Apoptosis in Primary Hepatic Carcinoma
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摘要: 目的探讨原发性肝癌早期凋亡相关蛋白M30的表达及其与凋亡的关系。方法采用免疫组织化学链霉素抗生物素蛋白-过氧化物酶（SP）法分别检测了80例原发性肝癌及相应的癌旁组织、10例正常肝组织M30蛋白的表达情况,采用脱氧核糖核酸末端转移酶介导的缺口末端标记（TUNEL）技术,检测肝癌凋亡指数。结果80例原发性肝癌、相应的癌旁组织、10例正常肝组织M30平均指数分别为9.10±7.05、3.31±3.02、0.10±0.32,肝癌组织M30指数和TNM分期、组织分化程度、生长方式、生存期及复发时间密切相关（P<0.05）。M30指数与凋亡指数呈显著正相关（P<0.01）。结论M30有可能成为对肝癌预后进行推测的分子标志之一。Abstract: Objective To study the expression of M30 protein in primary hepatic carcinoma and the relationship between M30 expression and apoptosis in the tumor.Methods The expression of M30 protein was examined with immunohistochemical S-P method in 80 PHC tissues and pericarcinomatous hepatic tissues and 10 normal 1iver tissues.The apoptosis index (AI) was examined with terminal deoxymucleotidyl transference enzyme mediated dUTP nick end labeling (TUNEL) technique.Results M30 index was 9.10±7.05、3.31±3.02、0.10±0.32 respectively in PHC tissues, pericarcinomatous hepatic tissues and normal 1iver tissues.M30 index of PHC was closely associated with TNM stage, histological differentiation, growth patten, survival and recurrent time (P<0.05) .The expression of M30 was positively correlated with the AI of PHC cell (P<0.01) .Conclusion M30 is possible to be an indicator of PHC prognosis.
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Key words:
- primary hepatic carcinoma /
- immunohistochemistry /
- M30 /
- apoptosis /
- prognosis
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[1]Edmondson HA, Steiner PE.Primary carcinoma of the liver-a studyof 100 case among 49800 necropsies[J].Cancer, 1954, 7∶462. [2]Leers MP, Kolgen W, Bjorklund V, et al.Immunocytochemical de-tection and mapping of a cytokeratin 18 neo-epitope exposed duringearly apoptosis[J].J Pathol, 1999, 187∶567-572. [3]吴玉新, 王靖华, 王华, 等.Ki-67、早期凋亡相关蛋白M30在子宫内膜癌的表达及其与预后的关系[J].中华病理学杂志, 2003, 32∶314-318. -
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