凋亡抑制因子Survivin表达在大鼠重症急性胰腺炎肾损伤中的作用

樊恒; 宋甫春; 包俊炜; 赵宇

凋亡抑制因子Survivin表达在大鼠重症急性胰腺炎肾损伤中的作用

1. 宁波大学医学院
2. 宁波市第一医院肝胆外科

基金项目:

宁波市医学科技计划项目(2009A13)；

详细信息

中图分类号: R657.5;R-332
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出版历程
- 出版日期: 2012-08-20

Survivin expression in kidney tissues of rats with severe acute pancreatitis complicated with renal injury

Research funding:

摘要

摘要: 目的研究大鼠重症急性胰腺炎(SAP)肾组织细胞间凋亡抑制因子Survivin表达与肾损伤的关系。方法 40只SD大鼠,体重(250±20)g,随机分为假手术组和重症急性胰腺炎6h、12 h、24 h组,每组10只。实验组采用胰胆管逆行穿刺注射4.5%牛磺胆酸钠(1.0 ml/kg)建立不同炎症程度的SAP模型,比较假手术组与各实验组胰腺组织和肾组织病理学变化,检查各组血清淀粉酶(AMY)、血肌酐(Cr)和血尿素氮(BUN),应用免疫组化方法检测胰腺和肾组织中Survivin的表达,肾脏组织细胞凋亡测定法(TUNEL法)检测肾组织细胞凋亡的变化。结果各实验组AMY、Cr、BUN较假手术组有所升高,Survivin在假手术组胰腺组织和肾组织表达均呈阴性,Survivin在SAP大鼠胰腺组织中的表达主要集中在胰腺导管处,腺泡细胞也有散在表达,Survivin在SAP大鼠肾组织中表达主要集中在肾小管和肾小球。肾小球细胞凋亡指数明显高于对照组[6、12、24 h分别为(3.10±1.00)vs(0.79±0.11),P<0.05;(4.01±1.90)vs(0.87±0.12),P<...
- 胰腺炎,急性坏死性 /
- 凋亡抑制蛋白质类 /
- Survivin /
- 肾功能衰竭,急性
Abstract: Objective To investigate the expression of Survivin in kidney tissues during severe acute pancreatitis (SAP) complicated with renal injury using a rat model. Methods Sprague-Dawley rats (weight: 230-270 g) were randomly divided into four groups (n=10 each) : control: sham operation;SAP induction: 6 h, 12 h, and 24 h post-infusion.Rats in the SAP groups were given 4.5% sodium taurocholate injection into the biliopancreatic duct.Severity of SAP complicated with renal injury was analyzed by detecting changes in serum amylase (AMY) , creatine (Cr) , blood urea nitrogen (BUN) , and histology of pancreas and kidney.Changes in Survivin protein expression were detected by immunohistochemistry.Changes in the apoptotic ratio of acinar cells in pancreas and kidney was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Results Serum AMY, Cr, and BUN were higher in SAP groups (vs sham operated group) .Survivin expression was negative in the sham operated group, but in the SAP groups was positive in both pancreatic tissue, mainly in ductal cells and a few acinar cells, and renal tissue, mainly in tubules and glomeruli.The apoptotic index of renal glomerular cells showed a time-dependent significant increase in SAP groups, compared to the sham-operated group [6 h: (3.10±1.00) vs (0.79±0.11) , P<0.05;12 h: (4.01±1.90) vs (0.87±0.12) , P<0.05;24 h: (5.00±1.80) vs (0.81±0.19) , P<0.01], as did the apoptotic index of renal tubule cells [6 h: (9.50±1.01) vs (1.29±0.47) , P<0.01; (11.90±2.30) vs (1.27±0.29) , P<0.01; (14.9±2.57) vs (1.21±0.32) , P<0.01].In general, the amount of apoptotic cells gradually increased over time in renal tissues of SAP groups. Conclusion Survivin is highly expressed in the pancreatic and kidney tissues of rats with severe acute pancreatitis complicated with renal injury.The level of altered Survivin expression may be related to the level of renal injury in acute pancreatitis, which may reflect a functional role in resolving kidney injury.
- pancreatitis /
- acute necrotizing /
- inhibitor of apoptosis proteins /
- Survivin

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参考文献(7)

[1]Aqarwal N, Pitchumoni CS.Acute pancreatitis:a multisystemdisease[J].Gastroenterologist, 1993, 1 (2) :115-128.

[2]朱明德, 方驰华, 史学深.重症胰腺炎肾损伤中细胞凋亡相关基因的作用[J].世界华人消化杂志, 2005, 13 (17) :2103-2107.

[3]靳风烁, 梁培禾, 岳明.大鼠急性肾功能衰竭时肾小管上皮细胞凋亡及其意义[J].中华创伤杂志, 2000, 16:529-532.

[4]程宝泉, 刘春涛, 钟宁, 等.重症急性胰腺炎急性肾损伤机制探讨[J].山东大学学报 (医学版) , 2007, 45 (2) :1-6.

[5]Mega S, Miyamoto M, Li L, et al.Immunohistochemical analy-sis of nuclear Survivin expression in esophageal squamous cellcarcinoma[J].Dis Esophagus, 2006, 19 (5) :355-359.

[6]Duffy MJ, O'Donovan N, Brennan DJ, et al.Survivin:apromising tumor biomarker[J].Cancer Lett, 2007, 249 (1) :49-60.

[7]Tashiro M, Nakamura H, Taguchi M, et al.Expression ofSurvivin after acute necrohemorrhagic pancreatitis in rats[J].Pancreas, 2003, 26 (2) :160-165.

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