非酒精性脂肪性肝病生物样本库的建立与管理

陈立震; 张海英; 姜曼; 牛同红; 王健; 耿宁; 刘昊刚; 辛永宁; 宣世英

doi:10.3969/j.issn.1001-5256.2014.09.017

非酒精性脂肪性肝病生物样本库的建立与管理

DOI: 10.3969/j.issn.1001-5256.2014.09.017

1. 青岛大学医学院
2. 青岛市中心医院消化内科
3. 青岛市消化疾病重点实验室
4. 青岛市市立医院消化内二科

基金项目:

国家自然科学基金资助项目(81170337/H0304)；

详细信息

中图分类号: R575.5
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- 被引次数: 0
出版历程
- 收稿日期: 2013-11-28
- 出版日期: 2014-09-20

Establishment and management of nonalcoholic fatty liver disease biobank

Qingdao University Medical College

Research funding:

摘要

摘要:
目的探讨非酒精性脂肪性肝病（NAFLD）血液标本的采集和保存及生物样本库的建立和信息化管理方法。方法收集2009年10月-2013年10月经B超及临床血液学检测诊断为NAFLD的1226例患者的全血标本,进行生化指标测定、血浆及全血基因组DNA提取,并检测DNA的浓度和纯度,分别放至-80℃超低温冰箱储存备用,同时建立一套信息管理系统用于NAFLD生物样本库的管理。结果 1226例NAFLD患者的全血标本,其中包括双生子标本83例及家系标本100例。血浆及DNA的提取成功率均为100%,并随机抽取100份DNA进行检测,结果表明所收集标本能够满足后续试验要求。结论该研究成功建立了NAFLD生物样本库,拥有标准化的信息管理系统,对标本进行质量控制和信息化管理,为进一步研究NAFLD奠定了良好的基础。
- 脂肪肝 /
- 生物标本库 /
- 信息存储和检索
Abstract:
Objective To investigate the collection and preservation of blood specimens from patients with nonalcoholic fatty liver disease ( NAFLD) and the establishment and information management of biobank. Methods Whole blood samples were collected from 1226 patients who were diagnosed with NAFLD based on B- mode ultrasound and blood tests from October 2009 to October 2013. Biochemical parameters were measured. Plasma and whole- blood genomic DNA was extracted from the samples, and the purity and concentration of DNA were determined. Specimens were preserved in a refrigerator (- 80℃) . An information management system for NAFLD biobank was established. Results Specimens of 1226 NAFLD patients, including those of 83 twins and 100 families, were collected. The success rate was100% for extraction of plasma and whole- blood genomic DNA. One hundred DNA samples were randomly selected for testing, and the results showed that the collected specimens met the requirements of following experiments. Conclusion The NAFLD Biobank has been successfully established in this study. It has the standard information management system and enables the quality control and information management of specimens, laying a solid foundation for further research on NAFLD.
- fatty liver /
- biological specimen banks /
- information storage and retrieval

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参考文献(11)

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