星形细胞上调基因1、β-连环素及周期素D1在肝细胞癌中的表达及临床意义

于营; 罗新华; 程明亮; 程云娟

doi:10.3969/j.issn.1001-5256.2016.08.023

星形细胞上调基因1、β-连环素及周期素D1在肝细胞癌中的表达及临床意义

DOI: 10.3969/j.issn.1001-5256.2016.08.023

1. 贵州医科大学感染病学教研室
2. 秦皇岛市第一医院感染科
3. 贵州省人民医院感染科

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中图分类号: R735.7
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出版历程
- 收稿日期: 2016-03-07
- 出版日期: 2016-08-20

Expression and clinical significance of astrocyte elevated gene-1,β-catenin,and cyclin D1 in hepatocellular carcinoma

Department of Infectious Diseases,Guizhou Medical University

摘要

摘要: 目的探讨肝细胞癌（HCC）组织中星形细胞上调基因（AEG）1、β-连环素（β-catenin）及周期素D1（Cyclin D1）的表达及临床意义。方法随机选取2013年7月-2014年12月贵州省人民医院经手术及病理证实为HCC的癌组织标本和对应癌旁组织各40例,另选取8例正常肝组织作为对照组。采用免疫组化SP法检测AEG-1、β-catenin及Cyclin D1蛋白在HCC组织、对应癌旁肝组织及正常肝组织中的表达情况,并分析其表达与HCC临床病理因素的相关性。计数资料组间比较采用χ2检验或Fisher确切概率法,AEG-1、β-catenin、Cyclin D1在HCC中的相关性采用Spearman等级相关分析。结果 AEG-1、β-catenin及Cyclin D1在HCC组织及癌旁肝组织中的表达均高于正常肝组织,差异均有统计学意义（χ2值分别为7.840、4.274、8.817、4.274、9.919、4.850,P值分别为0.005、0.039、0.003、0.039、0.002、0.028）。AEG-1、β-catenin、Cyclin D1的阳性表达在性别、年龄、HBs ...
- 癌,肝细胞 /
- 星形细胞上调基因1 /
- β连环素 /
- 细胞周期蛋白D1
Abstract: Objective To investigate the expression and clinical significance of astrocyte elevated gene- 1( AEG- 1),β- catenin,and cyclin D1 in hepatocellular carcinoma( HCC) tissues. Methods A total of 40 HCC samples and 40 samples of corresponding para- carcinoma tissues from the patients with pathologically confirmed HCC who underwent surgery in The People's Hospital of Guizhou from July 2013 to December 2014 were randomly selected,and 8 samples of normal liver tissues were selected as controls. The immunohistochemistry SP was used to measure the protein expression of AEG- 1,β- catenin,and cyclin D1 in HCC tissues,corresponding para- carcinoma tissues,and normal liver tissues,and the correlation between their expression and HCC clinicopathological characteristics was analyzed. The chi- square test or Fisher's exact test was used for comparison of categorical data between groups,and the Spearman rank correlation was used to analyze the correlation of AEG- 1 with β- catenin,and cyclin D1 in HCC. Results HCC tissues and para- carcinoma tissues showed significantly higher protein expression of AEG- 1,β- catenin,and cyclin D1 than normal liver tissues( χ2= 7. 840,4. 274,8. 817,4. 274,9. 919,and 4. 850,P = 0. 005,0. 039,0. 003,0. 039,0. 002,and 0. 028). The positive expression of AEG- 1,β- catenin,and cyclin D1 showed no significant differences across the patients with different sexes,ages,HBs Ag status,or tumor sizes( all P > 0. 05),but showed significant differences across the patients with different degrees of pathological differentiation,TNM stages for liver cancer,and metastases( all P < 0. 05). The correlation analysis showed that the protein expression of AEG- 1 was positively correlated with that of β- catenin and cyclin D1( r = 0. 420 and 0. 741,both P < 0. 01). Conclusion AEG- 1,β- catenin,and cyclin D1 may play vital roles in the development and progression of HCC. AEG- 1 may up- regulate the expression and activity of cyclin D1 and β- catenin and thus promote the development and metastasis of HCC. A combined measurement of AEG- 1,β- catenin,and cyclin D1 can be used as an important parameter for HCC gene therapy and prognostic evaluation.
- carcinoma /
- hepatocellular /
- astrocyte elevated gene 1 /
- beta catenin

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参考文献(19)

[1]ZHANG T,YANG J.Progress on elevated gene-1 in astrocyte[J].Basic Clin Med,2014,34(1):126-129.(in Chinese)张婷,杨军.星形细胞上调基因-1的研究进展[J].基础医学与临床,2014,34(1):126-129.

[2]ZHENG J,LI C,WU X,et al.Astrocyte elevated gene-1(AEG-1)shRNA sensitizes Huaier polysaccharide(HP)-induced anti-metastatic potency via inactivating downstream P13K/Akt pathway as well as augmenting cell-mediated immune response[J].Tumour Biol,2014,35(5):4219-4224.

[3]ZHENG J,LI C,WU X,et al.Huaier polysaccharides suppresses hepatocarcinoma MHCC97-H cell metastasis via inactivation of EMT and AEG-1 pathway[J].Int J Biol Macromol,2013,64(2):106-110.

[4]ZHENG J,LI C,WU X,et al.Astrocyte elevated gene-1 is a novel biomarker of epithelial-mesenchymal transition and progression of hepatocellular carcinoma in two China regions[J].Tumour Biol,2014,35(3):2265-2269.

[5]YOO BK,EMDAD L,LEE SG,et al.Astrocyte elevated gene-1(AEG-1):a multifunctional regulator of normal and abnormal physiology[J].Pharmacol Ther,2011,130(1):1-8.

[6]MUKHERJEE N,BHATTACHARYA N,ALAM N,et al.Subtype-specific alterations of the Wnt signaling pathway in breast cancer:clinical and prognostic significance[J].Cancer Sci,2012,103(2):210-220.

[7]LIU J,ZHANG MJ,ZHU QM,et al.Relationship between Wnt/β-catenin signaling pathway and hepatocellular carcinoma[J].Chin J Gastroenterol Hepatol,2014,23(9):994-997.(in Chinese)刘佳,张明娟,朱琴梅,等.Wnt/β-catenin信号通路在肝癌发病机制中作用的研究[J].胃肠病学和肝病学杂志,2014,23(9):994-997.

[8]ZHOU W,XIONG QR.Mechanisms of action of important molecules and target genes in the Wnt/β-catenin signaling pathway in primary liver cancer[J].J Hepatobiliary Surg,2013,21(2):150-155.(in Chinese)周威,熊奇如.Wnt/β-catenin信号通路重要分子及其靶基因在原发性肝癌中的作用机制[J].肝胆外科杂志,2013,21(2):150-155.

[9] JIAN-BO X,HUI W,YU-LONG H,et al.Astrocyte-elevated gene-1 overexpression is associated with poor prognosis in gastric cancer[J].Med Oncol,2011,28(2):455-462.

[10] EDMONDSON HA,STEINER PE.Primary carcinoma of the liver:a study of 100 cases among 48,900 necropsies[J].Cancer,1954,7(3):462-503.

[11]Ministry of Health of the People's Republic of China.Diagnosis,management,and treatment of hepatocellular carcinoma(V2011)[J].J Clin Hepatol,2011,27(11):1141-1159.(in Chinese)中华人民共和国卫生部.原发性肝癌诊疗规范(2011年版)[J].临床肝胆病杂志,2011,27(11):1141-1159.

[12]ZHANG JB,LI C,LI YH,et al.Study on the association between expression and clinical significance of AEG-1 and MMP-9 in epithelial ovarian carcinoma[J].Pract Oncol J,2013,27(3):234-238.(in Chinese)张静波,李聪,李英红,等.AEG-1和MMP-9在上皮性卵巢癌组织中的表达及其临床相关性和病理特征[J].实用肿瘤学杂志,2013,27(3):234-238.

[13] ANTTILA V,STEFANSSON H,KALLELA M,et al.Genome-wide association study of migraine implicates a common susceptibility variant on8q22.1[J].Nat Genet,2010,42(10):869-873.

[14] LIU X,ZHANG N,LI X,et al.Identification of novel variants of metadherin in breast cancer[J].PLo S One,2011,6(3):e17582.

[15] LI J,ZHANG N,SONG LB,et al.Astrocyte elevated gene-1 is a novel prognostic marker for breast cancer progression and overall patient survival[J].Clin Cancer Res,2008,14(11):3319-3326.

[16]SUN S,KE Z,WANG F,et al.Overexpression of astrocyte-elevated gene-1 is closely correlated with poor prognosis in human non-small cell lung cancer and mediates its metastasis through up-regulation of matrix metalloproteinase-9 expression[J].Hum Pathol,2012,43(7):1051-1060.

[17] THIRKETTLE HJ,MILLS IG,WHITAKER HC,et al.Nuclear LYRIC/AEG-1 interacts with PLZF and relieves PLZF-mediated repression[J].Oncogene,2009,28(41):3663-3670.

[18]SHEN CC,ZHU WG.Research progress ofβ-catenin post-translational modification and its function[J].Scientia Sinica(Vitae),2015,45(11):1110-1116.(in Chinese)申长春,朱卫国.β-catenin的翻译后修饰及其作用[J].中国科学:生命科学,2015,45(11):1110-1116.

[19]WAISBERG J,SABA GT.Wnt-/-β-catenin pathway signaling in human hepatocellular carcinoma[J].World J Hepatol,2015,7(26):2631-2635.

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