片仔癀对实验动物胆汁排泄的影响及镇痛作用观察

李俊峰; 任锋; 丁美; 刘霜; 陈志亮; 陈煜; 段钟平

doi:10.3969/j.issn.1001-5256.2019.03.023

片仔癀对实验动物胆汁排泄的影响及镇痛作用观察

DOI: 10.3969/j.issn.1001-5256.2019.03.023

1. 兰州大学第一医院传染病研究室&感染科
2. 首都医科大学附属北京佑安医院人工肝中心
3. 肝衰竭与人工肝治疗研究北京市重点实验室
4. 漳州片仔癀药业股份有限公司/福建省片仔癀天然医药研发企业重点实验室

基金项目:

北京市科技计划课题(Z171100002217070)；国家重点研发计划资助(2017YFA0103000)；国家科技重大专项艾滋病和病毒性肝炎等重大传染病防治(2012ZX10002004-006,2017ZX10203201-005,2017ZX10201201,2017ZX10202203-006-001,2017ZX10302201-004-002)；北京市医院管理局“登峰”人才培养计划基金资助项目(DFL20151601);北京市医院管理局临床医学发展专项经费资助(ZYLX201806)；国家自然科学基金(81770611,81800528)；福建省自然科学基金(2016J05210)；兰州大学第一医院院内基金(ldyyyn2017-17)；甘肃省卫生行业科研计划项目(GSWSKY2018-24)；兰州市城关区科研专项(2018SHFZ0023)；

详细信息

中图分类号: R285.5
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出版历程
- 出版日期: 2019-03-20

Effect of Pien Tze Huang on bile excretion and its analgesic effect in experimental animals

Institute of Infectious Diseases & Department of Infectious Diseases, The First Hospital of Lanzhou University

Research funding:

摘要

摘要:
目的观察片仔癀对实验动物胆汁排泄的影响及镇痛作用。方法将30只豚鼠随机分为片仔癀组、熊去氧胆酸组和对照组,根据体质量分别灌胃给予片仔癀（140 mg/kg）、熊去氧胆酸（22. 5 mg/kg）和生理盐水,连续4 d后行胆汁引流术,检测胆汁排泄量及胆汁成分变化,检测肝功能指标变化。将30只小鼠随机分组为片仔癀组（360 mg/kg）、熊去氧胆酸组（120 mg/kg）和对照组,连续给药4 d,末次给药1 h后,用0. 6%冰醋酸（0. 15 ml/10 g）腹腔注射,利用小鼠扭体实验观察片仔癀的镇痛作用。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果与对照组相比,片仔癀和熊去氧胆酸具有明显的促胆汁排泄作用（P值分别为0. 039和0. 009）。各组间胆汁中的主要成分:胆固醇、胆红素、总胆汁酸和磷脂比较,差异均无统计学意义（P值均> 0. 05）,各组肝功能指标:ALT、AST、GGT、TBil、DBil、IBil和TBA等亦均无统计学差异（P值均> 0. 05）。与对照组相比,片仔癀组小鼠扭体次数显著降低,具有显著的镇痛作用（P <0....
- 胆汁排泄 /
- 利胆药和促胆汁分泌药 /
- 镇痛 /
- 豚鼠 /
- 小鼠
Abstract:
Objective To investigate the effect of Pien Tze Huang on bile excretion and its analgesic effect in experimental animals. Meth-ods A total of 30 guinea pigs were randomly divided into Pien Tze Huang group, ursodeoxycholic acid group, and control group. Accordingto body mass, the three groups were treated with Pien Tze Huang ( 140 mg/kg) , ursodeoxycholic acid ( 22. 5 mg/kg) , and normal saline, respectively, by gavage for 4 consecutive days. Then bile drainage was performed to measure the volume of bile secretion and the change inbile composition. Related liver function parameters were also measured. A total of 30 mice were randomly divided into Pien Tze Huang group ( 360 mg/kg) , ursodeoxycholic acid group ( 120 mg/kg) , and control group. At one hour after the last administration, the mice were givenintraperitoneal injection of 0. 6% glacial acetic acid ( 0. 15 ml/10 g) , and the writhing test was performed to observe the analgesic effect ofPien Tze Huang. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least signifi-cant difference t-test was used for further comparison between two groups. Results Compared with the control group, the Pien Tze Huanggroup and the ursodeoxycholic acid group had a significant increase in the volume of bile secretion ( P = 0. 039 and 0. 009) . There were nosignificant differences between the three groups in cholesterol, bilirubin, total bile acid, and phospholipid in bile ( all P > 0. 05) and liverfunction parameters ( alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, total bilirubin, direct biliru-bin, indirect bilirubin, and total bile acid) ( all P > 0. 05) . Compared with the control group, the Pien Tze Huang group had a significantreduction in the number of writhing times ( P < 0. 001) , suggesting that Pien Tze Huang had a marked analgesic effect. Conclusion Ani-mal experiments show that Pien Tze Huang has marked choleretic and analgesic effects, which provides theoretical and data support for theclinical application of Pien Tze Huang.
- bile excretion /
- cholagogues and choleretics /
- analgesia /
- guinea pigs /
- mice

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参考文献(9)

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