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丙型肝炎核心抗原检测在丙型肝炎诊断中的意义
Detection of HCV core antigen and its diagnostic significance
文章发布日期:2013年01月15日  来源:  作者:杨杰,崔敬,刘春,等  点击次数:2070次  下载次数:551次

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【摘要】:目的了解丙型肝炎核心抗原(HCV-cAg)检测方法的敏感性及特异性,确定具有临床意义的S/CO值,探讨其在丙型肝炎诊断中的意义。方法使用ELASA方法检测丙型肝炎核心抗原,RT-PCR检测HCV RNA定量,观察不同S/CO值所对应的HCV RNA定量之间的关系,以HCV RNA为诊断金标准,列四格表做诊断实验。 结果HCV-cAg抗原检测的敏感性为87.05%,特异性为76.67%,阳性预测值为96.53%,阴性预测值为44.23%。结论(1)随着HCV-cAg的S/CO值逐渐增大,其与HCV RNA阳性符合率明显增高,随着HCV-cAg的S/CO值减小,其与HCV RNA阴性符合率明显增高; (2)S/CO值=2可以作为临床判断HCV感染病毒血症存在的一个标准;(3)本实验的敏感性和特异性较好,检测方法简单,可以作为丙型肝炎临床诊断的补充试验及筛查。
【Abstract】:ObjectiveTo compare the abilities of the hepatitis C virus (HCV) core antigen (cAg) test and the HCV RNA assay for confirming anti-HCV presence in order to determine the clinical utility of the HCV-cAg as an alternative or confirmatory diagnostic tool. Methods Serum samples collected from 158 patients diagnosed with HCV infection were subjected to the enzyme-linked immunosorbent assay-based HCV-cAg test. The optical density (OD) measured values were used to calculate the ratio of specimen absorbance to the cutoff value (S/CO). Simultaneously, the serum samples were subjected to PCR-based nucleic acid amplification quantitative fluorescence detection of HCV RNA. ResultsNone of the serum samples had a S/CO value <1 for the HCV-cAg test (100% negative), but all of the samples had a S/CO value >5 (100% positive). The HCV-cAg test sensitivity was 87.05%, specificity was 76.67%, positive predictive value was 9653%, and negative predictive value was 44.23%. As the S/CO value gradually increased, the significantly higher positive coincident rate of the HCV RNA test decreased. The HCV RNA negative coincident rate was significantly higher than that of the HCV-cAg test. HCV-cAg S/CO values between 1 and 2 corresponded to an HCV RNA values between 1.0×103 copies/ml and 1.0×104 copies/ml. The highest S/CO value obtained was 1.992. ConclusionThe HCV-cAg test is comparable to the HCV RNA assay for diagnosing HCV infection.
【关键字】:肝炎抗原,丙型;肝炎病毒属
【Key words】:hepatitis C antigen; hepacivirus
【引证本文】:

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