首 页   本刊简介  编委会  审稿专家  在线期刊  写作规范  广告合作  联系我们
您现在的位置:首页 => 在线期刊 => 2014年 10期 => 肝衰竭 =>雌激素受体α-29位基因多态性与..
雌激素受体α-29位基因多态性与甘肃地区汉族HBV相关慢加急性肝衰竭患者的关系
Association of ERα gene polymorphism at position 29 with HBV-related acute-on-chronic liver failure
文章发布日期:2014年09月12日  来源:  作者:周宁,张月荣,魏世芳,等  点击次数:2428次  下载次数:550次

调整字体大小:

(此处下载失败可以在在线预览处保存副本或者右键另存为)

【摘要】:目的了解雌激素受体(ER)α-29位多态性与HBV感染后果的关系,从基因水平上探讨HBV相关慢加急性肝衰竭(HBV-ACLF)的发病机制。方法收集2011年8月至2013年11月在兰州市第一人民医院住院及门诊就诊的甘肃籍汉族患者,采用聚合酶链反应-限制性片段长度多态性法检测61例HBV-ACLF患者、79例慢性乙型肝炎(CHB)患者、69例HBV感染自愈者的ERα-29位多态性。 用基因计数法计算检验人群的等位基因频率,进行Hardy-Weinberg遗传平衡定律检验。应用非条件Logistic回归分析将混杂因素进行调整。基因型及等位基因频率比较采用行×列的χ2检验。结果HBV-ACLF组TT基因型和T等位基因频率(55.7%,77.1%)明显高于CHB组患者(31.6%,54.4%),差异有统计学意义(χ2=8.194,P=0.004; χ2=15.341,P<0.001) ,携带T等位基因HBV感染重症化的风险是C等位基因的2.811倍(OR=2.811,95%CI:1.662~4.753)。HBV-ACLF组TT基因型和T等位基因频率(55.7%,77.1%)明显高于HBV感染恢复组患者(11.6%,326%),差异有统计学意义(χ2=28.849,P<0.001; χ2=51.401,P<0.001) ,T等位基因HBV感染重症化的风险是C等位基因的6.938倍(OR=6.938,95%CI:3.995~12.050)。结论ERα-29位TT基因型和T等位基因可能是HBV-ACLF的遗传易感基因。
【Abstract】:ObjectiveTo investigate the relationship between estrogen receptor alpha (ERα) gene polymorphism at position 29 and hepatitis B virus (HBV)-related acute-on-chronic liver failure (HBV-ACLF) and the genetic pathogenesis of HBV-ACLF. MethodsA total of 61 HBV-ACLF patients, 79 patients with chronic hepatitis B (CHB), and 69 patients who automatically recovered from HBV infection were included in the study. The ERα gene polymorphism at position 29 was analyzed by polymerase chain reaction-restriction fragment length polymorphism. The allele frequency was calculated by gene counting, and Hardy-Weinberg equilibrium test was performed. Confounding factors were adjusted by unconditional logistic regression analysis. Comparisons of genotype frequency and allele frequency were made by chi-square test (R×C). ResultsThe frequencies of TT genotype and T allele of ERα gene at position 29 were significantly higher in HBV-ACLF patients than in CHB patients (55.7% vs 31.6%, χ2=8.194, P=0.004; 77.1% vs 54.4%, χ2=15.341, P<0.001), and the risk of worsening in HBV infection patients with T allele was 2.811 times that in HBV infection patient with C allele (OR=2.811, 95%CI: 1.662-4.753). The frequencies of TT genotype and T allele of ERα gene at position 29 were significantly higher in HBV-ACLF patients than in patients who recovered from HBV infection (55.7% vs 11.6%, χ2=28.849, P<0.001; 77.1% vs 326%, χ2=51.401, P<0.001), and the risk of worsening in HBV infection patients with T allele was 6.938 times that in patients with C allele (OR=6.938, 95%CI: 3.995-12.050). Conclusion TT genotype and T allele of ERα gene at position 29 might be the susceptibility gene for HBV-ACLF.
【关键字】:肝功能衰竭;受体,雌激素;多态性,单核苷酸;多态性,限制性片段长度
【Key words】:liver failure; receptor, estrogen; polymorphisms, single nucleotide; polymorphisms, restriction fragment length
【引证本文】:

地址:长春市东民主大街519号《临床肝胆病杂志》编辑部 邮编:130061 电话:0431-88782542/3542
临床肝胆病杂志 版权所有 Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号

吉公网安备 22010402000041号