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Axin2在胰腺癌细胞中的表达及意义
Expression and significance of Axin2 in pancreatic cancer cells
文章发布日期:2016年04月07日  来源:  作者:张涛,陈国栋,贺更生,等  点击次数:1785次  下载次数:274次

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【摘要】:目的探讨Axin2在胰腺癌细胞中的表达情况及其对人胰腺癌细胞(PANC-1)增殖、侵袭及迁移能力的影响。方法采用实时荧光定量核酸扩增检测系统(QPCR)检测不同侵袭能力的胰腺癌细胞株(PANC-1、Mia PaCa-2、BxPC-3)和永生化正常胰腺细胞(H6C7)中Axin2表达情况;过表达Axin2质粒瞬时转染低表达的 PANC-1细胞株,采用MTT法、Transwell法及划痕实验分别检测转染过表达Axin2后细胞增殖、侵袭及迁移能力变化。多组间比较采用单因素方差分析,进一步两两比较采用SNK-q检验。结果Axin2在PANC-1、BxPC-3、Mia PaCa-2及H6C7细胞中相对表达量分别为0.13±0.01、0.42±0.05、0.24±0011和1.00±0.00,其中PANC-1细胞中表达水平最低,与其他组比较差异均有统计学意义(P值均<0.05);过表达Axin2载体转染 PANC-1细胞后,过表达组细胞中Axin2相对表达量与空白、阴性对照组相比,其表达量显著升高,差异均有统计学意义(P值均<0.05);与未转染组和空白对照组相比,过表达组的PANC-1细胞增殖、侵袭及迁移能力均明显减弱。结论Axin2在胰腺癌细胞株较正常胰腺细胞表达下调,在侵袭能力越高的癌细胞株中表达越低,提示起抑癌基因作用;Axin2高表达能减弱PANC-1细胞的增殖、迁移及侵袭能力。
【Abstract】:ObjectiveTo investigate the expression of Axin2 in pancreatic cancer cells, and to observe the influence of Axin2 on the proliferation, invasion, and migration of human pancreatic cancer cells (PANC-1). MethodsQuantitative real-time PCR was used to measure the expression of Axin2 in pancreatic cancer cell lines with different invasive abilities (PANC-1, Mia PaCa-2, and BxPC-3) and immortalized normal pancreatic cells (H6C7). PANC-1 cells with low expression were transfected with over-expressed Axin2 plasmid by transient transfection. MTT assay, Transwell assay, and scratch assay were used to determine the proliferation, invasion, and migration of cells transfected with over-expressed Axin2. One-way analysis of variance was used for comparison between multiple groups, and SNK-q test was used for comparison between any two groups. ResultsThe relative expression levels of Axin2 in PANC-1, BxPC-3, Mia PaCa-2, and H6C7 cells were 0.13±0.01, 0.42±0.05, 0.24±0.011, and 1.00±0.00, respectively, and PANC-1 cells had the lowest expression level of Axin2, with significant differences compared with the other cells (all P<0.05). When PANC-1 cells were transfected with over-expressed Axin2 plasmid, the cells in the over-expression group had a significant increase in the expression level of Axin2 compared with those in the blank group and the negative control group (both P<0.05). Compared with those in the non-transfection group and the blank group, PANC-1 cells in the over-expression group showed significant reductions in the proliferation, invasion, and migration abilities. ConclusionThe expression of Axin2 is down-regulated in pancreatic cancer cell lines and decreases with the increasing invasion ability, suggesting the role of tumor suppressor gene. High expression of Axin2 can reduce the proliferation, invasion, and migration abilities of PANC-1 cells.
【关键字】:胰腺肿瘤;肿瘤转移;肿瘤侵润;Axin2
【Key words】:pancreatic neoplasms;neoplasm metastasis;neoplasm invasiveness;Axin2
【引证本文】:张涛, 陈国栋, 贺更生, 等. Axin2在胰腺癌细胞中的表达及意义[J]. 临床肝胆病杂志, 2016, 32(5): 876-879.

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