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【摘要】：目的研究苦参碱的抗氧化作用，并观察其治疗非酒精性脂肪性肝炎（NASH）大鼠的疗效。方法30只大鼠随机分为3组：对照组、NASH组和苦参碱组，每组10只。采用高脂饮食诱导建立NASH大鼠模型，并给予苦参碱36 mg·kg-1·d-1灌胃治疗。观察所有大鼠体质量、肝重变化，并采用HE染色观察各组大鼠肝组织病理学改变，测定血清ALT、AST水平及肝组织还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）活性。多组间比较采用单因素方差分析，进一步两两比较采用SNK-q检验。结果与对照组比较，NASH组、苦参碱组的大鼠体质量均明显增加（P值均<0001），肝重均明显减轻（P值均<0001）。苦参碱组大鼠肝组织病理学改变较NASH组亦明显减轻。苦参碱组大鼠ALT、AST水平与NASH组比较均有所降低，差异均有统计学意义［（52.0±3.0) U/L vs(41.8±3.7) U/L；(233.6±9.4) U/L vs (170.1±1.8) U/L，P值均<0.001）］。苦参碱组与NASH组比较，大鼠肝组织SOD、GSH水平明显升高［（17.7±2.0）μg/mg vs（27.0±3.6）μg/mg;（16.5±1.6）U/mg vs（28.5±2.1）U/mg］，MDA水平明显降低［（22.9±1.9）nmol/mg vs（17.8±1.8）nmol/mg］，差异均有统计学意义（P值均<0.001）。结论苦参碱在治疗NASH大鼠中具有抗氧化的作用，对NASH大鼠的治疗作用显著。
【Abstract】：ObjectiveTo investigate the antioxidant effect of matrine and its clinical effect in the treatment of rats with nonalcoholic steatohepatitis (NASH). MethodsA total of 30 rats were randomly divided into three groups: control group, NASH group, and matrine group, with 10 in each. A high-fat diet was used to establish the rat model of NASH, and matrine was given by gavage for treatment at a dose of 36 mg·kg-１·d-1. The changes in body weight and liver weight were observed in all rats. HE staining was used to observe the histopathological changes of the liver. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the content of reduced glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA) in liver tissue were measured. A one-way analysis of variance was used for the comparison between multiple groups, and the SNK-q test was used for further comparison between any two groups. ResultsCompared with the NASH group, the matrine group had significant reductions in the serum levels of ALT and AST (ALT: 52.0±3.0 U/L vs 41.8±3.7 U/L, P<0.001; AST: 233.6±9.4 U/L vs 170.1±1.8 U/L, P<0.001). The matrine group showed marked improvement in the histopathological changes of the liver compared with the NASH group. Compared with the NASH group, the matrine group had significantly increased content of SOD and GSH in liver tissue (SOD: 17.7±2.0 μg/mg vs 27.0±3.6 μg/mg, P<0.001; GSH: 16.5±1.6 U/mg vs 28.5±2.1 U/mg, P<0.001) and significantly reduced content of MDA (22.9±1.9 nmol/mg vs 17.8±1.8 nmol/mg, P<0.001). ConclusionMatrine has an antioxidant effect and a marked clinical effect in the treatment of rats with NASH.
【关键字】：脂肪肝； 苦参碱； 抗氧化剂
【Key words】：fatty liver; matrine; antioxidants
【引证本文】：艾正琳， 谢步善， 姚树坤. 苦参碱治疗非酒精性脂肪性肝炎大鼠的效果观察［J］. 临床肝胆病杂志, 2016, 32(11): 2163-2166.