药对“川楝子-延胡索”治疗肝癌的网络药理学研究

章甜; 贾思静; 孙冬雪; 龙奉玺; 唐东昕; 杨柱

doi:10.3969/j.issn.1001-5256.2021.09.026

药对“川楝子-延胡索”治疗肝癌的网络药理学研究

DOI: 10.3969/j.issn.1001-5256.2021.09.026

1.
贵州中医药大学研究生院，贵阳 550025
2.
贵州中医药大学第一附属医院肿瘤科，贵阳 550001

基金项目:

中医肿瘤学研究生工作站 (JYSZ(2014)018);

贵州省高层次创新型人才培养计划(百层次) ((2016)4032);

贵州省中医肿瘤传承与科技创新人才基地 ((2018)3);

贵州省杨柱“中医肿瘤学”研究生导师工作室 (GZS(2016)08);

贵阳市科技局贵州中医药大学第一附属医院大健康科技合作项目 ((2019)9-2);

贵州省科技厅贵州省中医肿瘤传承与科技创新人才团队 ((2020)5013);

国家自然科学基金 (81660833);

贵州中医药大学研究生教育创新计划 (YFYYJSCX2018-28)

利益冲突声明：本研究不存在研究者、伦理委员会成员、受试者监护人以及与公开研究成果有关的利益冲突。

作者贡献声明：章甜负责课题设计、拟定写作思路、撰写论文；贾思静、孙冬雪负责收集数据及资料分析；龙奉玺、杨柱负责修改论文；龙奉玺、唐东昕指导撰写文章并最后定稿。

详细信息

通信作者:
龙奉玺，1343750581@qq.com

中图分类号: R735.7
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- 被引次数: 0
出版历程
- 收稿日期: 2021-02-06
- 录用日期: 2021-03-09
- 出版日期: 2021-09-20

Mechanism of "Szechwan Chinaberry Fruit-Rhizoma Corydalis" drug combination in treatment of liver cancer based on network pharmacology

1.
Graduate School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
2.
Department of Oncology, The First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550001, China

Research funding:

Postgraduate Workstation of TCM Oncology (JYSZ(2014)018);

High-level Innovative Talent Cultivation Plan of Guizhou Province (Hundred Levels) ((2016)4032);

Guizhou Provincial Talent Base for TCM Tumor Inheritance and Science and technology Innovation ((2018)3);

Yang Zhu "Traditional Chinese Medicine Oncology" Graduate Tutor Studio, Guizhou Province (GZS(2016)08);

Large health science and technology Cooperation project of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang Science and Technology Bureau ((2019)9-2);

Guizhou Provincial Department of Science and Technology of Traditional Chinese Medicine Tumor Inheritance and Science and Technology Innovation Talent Team ((2020)5013);

National Natural Science Foundation of China (81660833);

Graduate Education Innovation Program of Guizhou University of Traditional Chinese Medicine (YFYYJSCX2018-28)

摘要

摘要: 目的基于网络药理学方法研究药对“川楝子-延胡索”药理成分，分析该药对治疗肝癌的潜在分子机制。方法借助相关数据库如TCMSP、Uniprot、Genecard等分别搜集中药川楝子、延胡索的有效成分、对应作用靶点以及疾病肝癌的靶点，选取药物、疾病交集靶点。此外，通过String、Metascape数据库筛选药物作用核心靶点并完成GO功能和KEGG通路富集分析。结果中药川楝子、延胡索的有效成分分别为6、49个，共同成分1个；作用靶点分别为181、1097个，共同靶点143个；药对与肝癌的交集靶点162个，主要涉及的基因为IL6、TP53、VEGFA、TNF、CASP3；KEGG分析主要涉及通路为癌症通路、糖尿病并发症的AGE-RAGE信号通路、TNF信号通路、NF-κB信号通路、甲状腺激素信号通路等。结论药对“川楝子-延胡索”中含有多种不同成分，该成分可通过作用于相关基因及信号通路发挥治疗肝癌的作用。
- 肝肿瘤 /
- 药对 /
- 川楝子 /
- 延胡索
Abstract: Objective To investigate the pharmacological components of "Szechwan Chinaberry Fruit-Rhizoma Corydalis" drug combination and its potential molecular mechanism in the treatment of liver cancer based on network pharmacology. Methods Related databases, such as TCMSP, Uniprot, and GeneCard, were used to obtain the effective components of Szechwan Chinaberry Fruit and Rhizoma Corydalis, their corresponding action targets, and the disease targets of liver cancer, and the intersecting targets of drugs and diseases were selected. In addition, STRING and Metascape databases were used to screen out the core targets of drug action and perform GO function and KEGG pathway enrichment analyses. Results There were 9 active components in Szechwan Chinaberry Fruit and 49 active components in Rhizoma Corydalis, with 1 common component between the two drugs; there were 181 action targets of Szechwan Chinaberry Fruit and 1097 action targets of Rhizoma Corydalis, with 143 common targets between the two drugs. There were 162 intersecting targets between the drug combination and liver cancer, and the main genes involved were IL6, TP53, VEGFA, TNF, and CASP3. KEGG analysis showed that the main pathways involved included cancer pathway, AGE-RAGE signaling pathway of diabetes complications, TNF signaling pathway, NF-κB signaling pathway, and thyroid hormone signaling pathway. Conclusion There are many different components in the drug combination of "Szechwan Chinaberry Fruit-Rhizoma Corydalis", which can exert a therapeutic effect on liver cancer by acting on related genes and signaling pathways.
- Liver Neoplasms /
- Paired Drugs /
- Fructus Toosendan /
- Corydalis Yanhusuo

HTML全文

图 1 药对“川楝子-延胡索”治疗肝癌的靶点基因的GO功能分析(含BP、MF、CC)

下载: 全尺寸图片幻灯片

图 2 药对“川楝子-延胡索”治疗肝癌的靶点基因的KEGG信号通路分析

下载: 全尺寸图片幻灯片

表 1 药对“川楝子-延胡索”中中药活性成分详情

中药	简称	MOL ID	分子名称	OB(%)	DL(%)
川楝子(简称：CLZ)	CLZ1	MOL001494	Mandenol	42.00	0.19
	CLZ2	MOL001495	Ethyl linolenate	46.10	0.20
	CLZ3	MOL002045	Stigmasterol	43.41	0.76
	CLZ4	MOL002056	(E)-3-[(2S, 3R)-2-(4-hydroxy-3-methoxy-phenyl)-7-methoxy-3-methylol-2, 3-dihydrobenzofuran-5-yl]acrolein	54.74	0.40
	CLZ5	MOL002058	Medioresil	57.20	0.62
延胡索(简称：YHS)	YHS1	MOL001454	Berberine	36.86	0.78
	YHS2	MOL001458	Coptisine	30.67	0.86
	YHS3	MOL001460	Cryptopin	78.74	0.72
	YHS4	MOL001461	Dihydrochelerythrine	32.73	0.81
	YHS5	MOL001463	Dihydrosanguinarine	59.31	0.86
	YHS6	MOL001474	Sanguinarine	37.81	0.86
	YHS7	MOL000217	(S)-Scoulerine	32.28	0.54
	YHS8	MOL002670	Cavidine	35.64	0.81
	YHS9	MOL002903	(R)-Canadine	55.37	0.77
	YHS10	MOL000359	Sitosterol	36.91	0.75
	YHS11	MOL004071	Hyndarin	73.94	0.64
	YHS12	MOL004190	(-)-alpha-N-methylcanadine	45.06	0.80
	YHS13	MOL004191	Capaurine	62.91	0.69
	YHS14	MOL004193	Clarkeanidine	86.65	0.54
	YHS15	MOL004195	Corydaline	65.84	0.68
	YHS16	MOL004196	Corydalmine	52.50	0.59
	YHS17	MOL004197	Corydine	37.16	0.55
	YHS18	MOL004198	Corynoline	46.06	0.85
	YHS19	MOL004199	Corynoloxine	38.12	0.60
	YHS20	MOL004200	Methyl-[2-(3, 4, 6, 7-tetramethoxy-1-phenanthryl)ethyl]amine	61.15	0.44
	YHS21	MOL004202	Dehydrocavidine	38.99	0.81
	YHS22	MOL004203	Dehydrocorybulbine	46.97	0.63
	YHS23	MOL004204	Dehydrocorydaline	41.98	0.68
	YHS24	MOL004205	Dehydrocorydalmine	43.90	0.59
	YHS25	MOL004208	Demethylcorydalmatine	38.99	0.54
	YHS26	MOL004209	13-methyldehydrocorydalmine	35.94	0.63
	YHS27	MOL004210	(1S, 8′R)-6, 7-dimethoxy-2-methylspiro[3, 4-dihydroisoquinoline-1, 7′-6, 8-dihydrocyclopenta [g]^{[1, 3]}benzodioxole]-8′-ol	43.95	0.72
	YHS28	MOL004763	Izoteolin	39.53	0.51
	YHS29	MOL004214	Isocorybulbine	40.18	0.66
	YHS30	MOL004215	leonticine	45.79	0.26
	YHS31	MOL004216	13-methylpalmatrubine	40.97	0.63
	YHS32	MOL004220	N-methyllaurotetanine	41.62	0.56
	YHS33	MOL004221	Norglaucing	30.35	0.56
	YHS34	MOL004224	Pontevedrine	30.28	0.71
	YHS35	MOL004225	Pseudocoptisine	38.97	0.86
	YHS36	MOL004226	Pseudoprotopine	53.75	0.83
	YHS37	MOL004228	Saulatine	42.74	0.79
	YHS38	MOL004230	Stylopine	48.25	0.85
	YHS39	MOL004231	Tetrahydrocorysamine	34.17	0.86
	YHS40	MOL004232	Tetrahydroprotopapaverine	57.28	0.33
	YHS41	MOL004233	Thaliporphine	31.87	0.56
	YHS42	MOL004234	2, 3, 9, 10-tetramethoxy-13-methyl-5, 6- dihydroisoquinolino[2, 1-b]isoquinolin-8-one	76.77	0.73
	YHS43	MOL000449	Stigmasterol	43.83	0.76
	YHS44	MOL000785	Palmatine	64.60	0.65
	YHS45	MOL000787	Fumarine	59.26	0.83
	YHS46	MOL000790	Isocorypalmine	35.77	0.59
	YHS47	MOL000791	Bicuculline	69.67	0.88
	YHS48	MOL000793	Bulbocapnine	47.54	0.69
共同成分	A1	MOL000098	Quercetin	46.43	0.28
注：简称用于Cytocape软件制图。

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