犀黄丸诱导Bel-7402细胞凋亡及其细胞内钙离子浓度的变化

李莉芳; 陈如山; 刘新民

犀黄丸诱导Bel-7402细胞凋亡及其细胞内钙离子浓度的变化

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2222
- HTML全文浏览量: 13
- PDF下载量: 867
- 被引次数: 0
出版历程
- 出版日期: 2003-06-20

Xihuangwan induce Bel-7402 cell apoptosis and the change of intracellular-free calcium level in the process

摘要

摘要: 研究犀黄丸（含药血清）诱导肝癌Bel- 74 0 2细胞凋亡的作用及凋亡过程中细胞内游离钙离子浓度的变化。DNA琼脂糖凝胶电泳法检测细胞凋亡 ,流式细胞仪检测凋亡过程中细胞内游离钙离子浓度的变化。DNA琼脂糖凝胶电泳法显示有明显的DNA梯带 ,同时在细胞凋亡过程中细胞内游离钙离子浓度明显升高 ,以凋亡早期较为明显。犀黄丸能诱导肝癌细胞凋亡 ,细胞内游离钙离子浓度升高可能是其机制之一
- 肝肿瘤/中医药疗法 /
- 细胞凋亡 /
- 钙离子 /
- 犀黄丸
Abstract: To study whether herbal medicinal mixture Xihuangwan can induced apoptosis of Bel-7402 cells in vitro and the dynamic change of intracellular-free calcium level ( i) .Apoptosis induced by serum containing Xihuangwan was investigated by agarose gel electrophoresis. Flow cytometry was used to measure the change of intracellular-free calcium level ( i) .After treatment with Xihuangwan, nuclear DNA of the Bel-7402 cells exhibited DNA ladder. i was significantly higher than that in control groups, especially at the beginning stage of apoptosis.Xihuangwan may be able to induce apoptosis of Bel-7402 cell line. The increase of i might be one of the mechanisms of Bel-7402 cellar apoptosis.
- hepatoma / treatment of TCM /
- cell apoptosis /
- calcium /
- xihuangwan

HTML全文

参考文献(5)

[1] 田代真一.“血清药理学”ど“血清药化学”-汉方の药理学かろ始まつた药物浓度测定の新しい世界[J].MP研究, 1988, (5) ∶54.

[2]刘海成.中药复方体外药理研究思考[J].中药新药与临床药理, 2000, 11 (1) ∶54.

[3]ColeKA , KohnEC .Calcium-mediatedsignaltranslation:biology, bio chemistryandtherapy[J].CancerMetastasisRev, 1994, 13∶33-41.

[4]BerridgeMJ.Inositoltrisphosphateandcalciumsignaling[J].Nature, 1993, 361∶315-325.

[5]ShulzL .ModulationofintracellularfreeCa2+ concentrationbyIP3-sen sitiveandIP3-insensitivenonmitochondrialCa2+ pool[J].CellSalcium, 1989, 10∶325-336.

施引文献

资源附件(0)

访问统计