脐血来源和慢性乙型肝炎患者自体细胞因子诱导的杀伤细胞体外增殖及杀伤活性的对比观察

刘力伟; 李嘉嘉; 朱立新; 刘波

doi:10.3969/j.issn.1001-5256.2014.09.009

脐血来源和慢性乙型肝炎患者自体细胞因子诱导的杀伤细胞体外增殖及杀伤活性的对比观察

DOI: 10.3969/j.issn.1001-5256.2014.09.009

1. 解放军第一○五医院生物治疗中心
2. 安徽医科大学附属第一医院中心实验室

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中图分类号: R512.62
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出版历程
- 收稿日期: 2013-12-23
- 出版日期: 2014-09-20

In vitro proliferation capacity and killing efficacy of CIK cells from umbilical cord blood versus patients with chronic hepatitis B

Center for Biological Therapy, 105 Hospital of PLA

摘要

摘要:
目的观察脐血和不同年龄段慢性乙型肝炎(CHB)患者细胞因子诱导的杀伤细胞(CIK细胞)的体外增殖能力及杀伤活性的特点。方法采集2012年1月至2012年12月在解放军第一○五医院分娩的健康胎儿的脐带血5份(A组);采集20例CHB患者的外周血,分为2组,即B组12例(年龄20~35岁),C组8例(年龄>35岁)。采用Ficoll两步分离法分离出单个核细胞,在体外应用细胞因子诱导培养成CIK细胞,检测CIK细胞的免疫表型,噻唑蓝比色法测定其对白血病K562细胞的杀伤活性,采用t检验分析不同组CIK细胞增殖倍数,卡方检验分析杀伤活性率的差异。结果第15天时,B组体外CIK细胞增殖达到(589.15±237.60)倍,A组为(600.93±249.10)倍,2组相比差异无统计学意义(P>0.05);而C组为(370.45±165.2)倍,与A、B组相比差异有统计学意义(P<0.05)。A、B、C 3组CIK细胞对K562的杀伤率分别为(77.3±5.1)%、(54.5±3.5)%、(44.1±3.4)%,A组与B、C 2组相比差异具有统计学意义(P<0.05、P<...
- 肝炎,乙型,慢性 /
- 胎血 /
- 杀伤细胞 /
- 细胞增殖 /
- 免疫活性
Abstract:
Objective To observe the in vitro proliferation capacity and killing efficacy of cytokine- induced killer ( CIK) cells from umbilical cord blood ( UCB) and versus chronic hepatitis B ( CHB) patients of different ages. Methods Five samples of UCB from healthy fetuses born in our hospital from January to December, 2012, as well as 20 samples of peripheral blood from CHB patients, were divided into group A ( 5 samples of UCB) , group B ( 12 samples of peripheral blood from CHB patients aged 20- 35 years) , and group C ( 8 samples of peripheral blood from CHB patients aged over 35 years) . Mononuclear cells were separated by Ficoll- Hypaque centrifugation. Peripheral blood mononuclear cells were induced into CIK cells in the presence of cytokines. The immunophenotypes of CIK cells were determined by flow cytometry. The killing efficacy of CIK cells against K562 cells was measured by MTT assay. Results On day 15, CIK cells from group B multiplied 589. 15 ± 237. 6 times, versus 600. 93 ± 249. 1 times for group A ( P > 0. 05) ; CIK cells from group C multiplied 370. 45 ±165. 2 times, which were significantly lower than those for groups A and B ( P < 0. 05) . The killing efficacy of CIK cells from groups A, B, and C against K562 cells was ( 77. 3 ± 5. 1) %, ( 54. 5 ± 3. 5) %, and ( 44. 1 ± 3. 4) %, respectively; there were significant differences in killing efficacy between group A and groups B and C ( P < 0. 01) . Conclusion UCB- derived CIK cells have high in vitro proliferation capacity and killing efficacy. The in vitro proliferation capacity of CIK cells declines in CHB patients aged over 35 years, so autologous transplantation of CIK cells is not suitable for this group of patients.
- hepatitis B, chronic /
- fetal blood /
- killer cells /
- cell proliferation /
- immunocompetence

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参考文献(12)

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