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青岛地区汉族人群葡萄糖激酶调节蛋白基因rs780094(C>T)位点多态性与非酒精性脂肪性肝病的相关性分析
DOI: 10.3969/j.issn.1001-5256.2015.07.022
Association between GCKR gene rs780094( C > T) polymorphism and non- alcoholic fatty liver disease in Han population in Qingdao,China
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摘要:
目的探讨葡萄糖激酶调节蛋白（GCKR）基因rs780094（C>T）位点多态性与青岛地区汉族人群非酒精性脂肪性肝病（NAFLD）的相关性。方法采用病例-对照分析对160例NAFLD患者和172例正常人的血液标本进行分析,采用PCR及基因型检测方法对变异位点进行基因检测,比较各组临床资料、基因型频率、等位基因频率等。计量资料组间比较采用t检验,计数资料组间比较采用χ2检验,非条件Logistic回归模型计算rs780094位点多态性与NAFLD发生的相对风险度。结果两组间年龄差异具有统计学意义（P=0.000）,各等位基因频率及基因型频率分布差异均无统计学意义（P值均>0.05）;T等位基因携带者患NAFLD的风险是C等位基因的1.068倍,差异无统计学意义（P>0.05）。经混杂因素校正后,Logistic回归模型分析结果显示与CC纯合子携带者相比,携带等位基因T的TT+CT基因型未增加NAFLD的发生风险（P>0.05）。结论青岛地区汉族人群中GCKR基因rs780094 T等位基因未增加患NAFLD的风险性。
Abstract:Objective To investigate the association between glucokinase regulatory protein( GCKR) gene rs780094( C > T) polymorphism and non- alcoholic fatty liver disease( NAFLD) in the Han population in Qingdao,China. Methods A case- control study was conducted in 180 patients with NAFLD and 172 healthy controls. Blood samples were collected from all the subjects and were genotyped for rs780094 by polymerase chain reaction and DNA sequencing method. Genotype and allele frequencies were compared between the control and case groups. Results No significant differences between the two groups were observed in both genotype and allele frequencies( both P > 0. 05).The variant GCKR rs780094 T allele did not significantly increase the risk of NAFLD( OR = 1. 068,95% CI: 0. 748- 1. 525,P = 0. 719)compared with the C allele. Logistic regression model analysis adjusted for confounding factors exhibited that the TT + CT genotype of GCKR rs780094 did not significantly increase the risk of NAFLD relative to the CC genotype( P > 0. 05). Conclusion GCKR rs780094 T allele may be not associated with the increased risk of NAFLD in the Han population in Qingdao.
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