microRNA-520a与HBV相关肝细胞癌临床病理学特征的相关性分析

张玉睿

doi:10.3969/j.issn.1001-5256.2017.04.018

microRNA-520a与HBV相关肝细胞癌临床病理学特征的相关性分析

DOI: 10.3969/j.issn.1001-5256.2017.04.018

张玉睿

泰山医学院附属医院感染科

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中图分类号: R735.7
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出版历程
- 出版日期: 2017-04-20

Correlation between microRNA-520a and clinicopathological features of HBV-related hepatocellular carcinoma

Zhang YuRui

Department of Infectious Diseases, The Affiliated Hospital of Taishan Medical College

摘要

摘要: 目的旨在评估microRNA-520a与肝细胞癌（HCC）患者临床病理参数之间的关系。方法收集2010年1月-2015年12月于泰山医学院附属医院行手术治疗的183例HCC患者病例资料及组织标本。定量Real-time PCR检测microRNA-520a在纳入患者的HCC组织及其对应的癌旁组织中的表达水平,并分析其表达水平与HCC患者的临床病理特征之间的关系。多组间比较采用单因素方差分析,2组间比较采用独立样本t检验。相关性分析采用Spearman相关分析法。结果 microRNA-520a在HCC组织中的表达水平,与其对应相邻的癌旁组织相比显著降低（1.92±0.76 vs 2.73±1.15,t=-6.731,P<0.001）。受试者工作特征曲线下面积为0.711（95%可信区间:0.640.79,P<0.001）。存在远期转移（P=0.031）、高临床TNM分期（P=0.022）、血清HBV阳性（P<0.001）、存在肿瘤分隔（P=0.026）的患者,其HCC组织中microRNA-520a的表达水平显著降低。相关因素分析表明,micro...
- 癌,肝细胞 /
- 微RNAs /
- 病理状态,体征和症状
Abstract: Objective To investigate the correlation between microRNA-520 a and clinicopathological parameters in patients with hepatocellular carcinoma (HCC) .Methods The medical records and tissue specimens of 183 HCC patients, who received surgical treatment in the Affiliated Hospital of Taishan Medical College from January 2010 to December 2015, were collected.Quantitative real-time PCR was used to measure the expression of microRNA-520 a in HCC tissues and corresponding adjacent tissues from 183 patients with HCC, and its correlation with clinicopathological parameters was analyzed.A one-way analysis of variance was used for comparison between multiple groups, and the independent samples t-test was used for comparison between two groups; Spearman correlation analysis was also performed.Results HCC tissues had significantly lower expression of microRNA-520 a than corresponding adjacent tissues (1.92 ± 0.76 vs 2.73 ±1.15, t=-6.731, P<0.001) .The area under the receiver operating characteristic (ROC) curve was 0.711 (95% CI:0.64-0.79, P<0.001) .There were significant reductions in the expression of microRNA-520 a in HCC tissues in patients with distant metastasis (P=0.031) , advanced TNM stage (P=0.022) , positive serum hepatitis B virus (HBV) (P<0.001) , or tumor separation (P=0.026) .The Spearman correlation analysis showed that the expression of microRNA-520 a was significantly correlated with HBV infection (r=-0.351, P<0.001) and tumor separation (r=-0.207, P=0.044) .Conclusion The expression of microRNA-520 a is significantly downregulated in HCC tissues and it may inhibit the progression of HCC.
- carcinoma, hepatocellular /
- microRNAs /
- pathological conditions, signs and symptoms

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