中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 11
Nov.  2013
Turn off MathJax
Article Contents

Roles of indocyanine green clearance test and 13C- methacetin breath test in assessing liver function in acute liver injury: a comparative study

DOI: 10.3969/j.issn.1001-5256.2013.11.016
Research funding:

 

  • Received Date: 2013-03-20
  • Published Date: 2013-11-20
  • Objective To conduct a comparative study of indocyanine green ( ICG) clearance test and13C- methacetin breath test (13C- MBT) in assessing the liver function in acute liver injury. Methods Forty- eight male Sprague- Dawley rats were randomly divided into control group and 6, 12, 24, 48, and 72 h model groups. The rats in control group were intraperitoneally injected with saline, while the rats in model groups were intraperitoneally injected with D- galactosamine ( 450 mg / kg) to establish an acute liver injury model. The pathological score of liver tissue, ICG retention rate at 15 minutes ( ICG R15) , and parameters of13C- MBT, such as duration to peak of13CO2delta over baseline ( DOB) curve ( T max) , maximum metabolization velocity ( MV max) , cumulative excretion at 120 min ( CUM120) , and maximum DOB ( DOB max) were monitored.Comparison between groups was made by one- way analysis of variance; pairwise comparison of means between samples was made by SNK- q test;correlation between two variables was analyzed by Pearson's correlation test. Results Compared with the control group, all model groups showed significantly increased pathological scores ( P < 0. 05) and significantly decreased MV max, CUM120, and DOB max ( P <0.05) . Pathological score was positively correlated with T max ( r =0.593, P <0.05) and negatively correlated with MV max, CUM120, and DOB max ( r =-0.731, -0.618, and-0.592, P < 0. 05 for all) . Compared with the control group, the 12, 24, and 48 h model groups had significantly increased ICG R15 ( P < 0. 05) , but there was no significant difference in ICG R15 between the 6, 12 h model group and control group ( P > 0. 05) . Conclusion Both13C- MBT and ICG clearance test can be used as the effective tools for monitoring the liver function in acute liver injury.13C- MBT is more sensitive than ICG clearance test in the early diagnosis of acute liver injury.

     

  • loading
  • [1]DUAN ZJ, DUAN SS, XU J, et al.The value of TBA with liver enzymology joint detection in liver disease diagnosis[J].Int J Lab Med, 2011, 32 (5) :612-613. (in Chinese) 段正军, 段生寿, 徐杰, 等.血清总胆汁酸与肝脏功能酶学指标联合检测在肝脏疾病诊断中的应用价值[J].国际检验医学杂志, 2011, 32 (5) :612-613.[2]GUPTA S, CHAWLA Y, KAUR J, et al.Indocyanine green clearance test (using spectrophotometry) and its correlation with model for end stage liver disease (MELD) score in Indian patients with cirrhosis of liver[J].Trop Gastroenterol, 2012, 33 (2) :129-134.[3]REN Z, XU Y, ZHU S.Indocyanine green retention test avoiding liver failure after hepatectomy for hepatolithiasis[J].Hepatogastroenterology, 2012, 59 (115) :782-784.[4]LEVESQUE E, HOTI E, AZOULAY D, et al.Non-invasive ICG-clearance:a useful tool for the management of hepatic artery thrombosis following liver transplantation[J].Clin Transplant, 2011, 25 (2) :297-301.[5]LIU F, XIANG HL, HAN T, et al.Clinical value of13C-methacetin breath test for assessing liver function in patients with cirrhosis[J].J Clin Hepatol, 2012, 28 (9) :661-666. (in Chinese) 刘芳, 向慧玲, 韩涛, 等.IRIS13C-美沙西汀呼气试验与Child-Pugh分级、MELD评分评估肝硬化患者肝功能的比较[J].临床肝胆病杂志, 2012, 28 (9) :661-666.[6]ZHANG L, KANG W, LEI Y, et al.Granulocyte colony-stimulating factor treatment ameliorates liver injury and improves survival in rats with D-galactosamine-induced acute liver failure[J].Toxicol Lett, 2011, 204 (1) :92-99.[7]CHEN Y.Functional assessment and prognosis of liver disease[J].J Clin Hepatol, 2010, 26 (6) :565-567, 584. (in Chinese) 陈煜.肝功能评价指标及预后评估[J].临床肝胆病杂志, 2010, 26 (6) :565-567, 584.[8]SHALEV T, AEED H, SORIN V, et al.Evaluation of the13C-octanoate breath test as a surrogate marker of liver damage in animal models[J].Dig Dis Sci, 2010, 55 (6) :1589-1598.[9]SHTEYER E, LALAZAR G, HEMED N, et al.Continuous13Cmethacetin breath test differentiates biliary atresia from other causes of neonatal cholestasis[J].J Pediatr Gastroenterol Nutr, 2013, 56 (1) :60-65.[10]AFOLABI P, WRIGHT M, WOOTTON SA, et al.Clinical utility of13C-liver-function breath tests for assessment of hepatic function[J].Dig Dis Sci, 2013, 58 (1) :33-41.[11]LANG ZW.The value of pathology detection in diagnosis and treatment of chronic hepatitis B[J].World Chin J Dig, 2003, 11 (6) :772-774. (in Chinese) 朗振为.病理学检测在慢性乙型肝炎诊断治疗中的价值[J].世界华人消化杂志, 2003, 11 (6) :772-774.[12]KNODELL RG, ISHAK KG, BLACK WC, et al.Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis[J].Hepatology, 1981, 1 (5) :431-435.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2429) PDF downloads(499) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return