Effect of sorafenib combined with thalidomide on angiogenesis in chick chorioallantoic membrane

Objective To investigate the effect of sorafenib and / or thalidomide on angiogenesis in chick chorioallantoic membrane( CAM).Methods White eggs incubated for 7 days were used to establish a CAM model. The model eggs were randomly divided blank control group,sorafenib group,thalidomide group,and sorafenib / thalidomide group. After treatment,each egg was incubated for another 2 days.The CAM samples were collected and fixed to take their pictures under a microscope,and the vascular coverage of each sample was calculated.Comparison between these groups was made by analysis of variance,and comparison between each two groups was made by SNK- q test.Results The thalidomide group or sorafenib group had significantly lower vascular coverage than the blank control group( 30. 2% ± 2. 9%or 26. 5% ± 2. 1% vs 38. 3% ± 2. 7%,P < 0. 05). The sorafenib / thalidomide group had significantly lower vascular coverage than the thalidomide group or sorafenib group( 12. 6% ± 1. 5% vs 30. 2% ± 2. 9% or 26. 5% ± 2. 1%,P < 0. 05). Conclusion Both sorafenib and thalidomide have a good anti- angiogenic effect on CAM,but a combination of the two drugs shows better efficacy.