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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 7
Jul.  2017
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Characteristics and clinical significance of peripheral follicular cytotoxic T cells in patients experiencing acute rejection after liver transplantation

DOI: 10.3969/j.issn.1001-5256.2017.07.027
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  • Received Date: 2017-01-12
  • Published Date: 2017-07-20
  • Objective To investigate the expression characteristics of peripheral follicular cytotoxic T (Tfc) cells in patients undergoing liver transplantation and their significance in acute rejection.Methods A total of 36 patients who underwent liver transplantation in Liver Transplantation Research Center in 302 Hospital of PLA from December 2015 to February 2017 were enrolled, and among these patients, 11 experienced acute rejection (AR group) and 25 did not experience acute rejection (NAR group) .A confirmed diagnosis of acute rejection was made by pathological diagnosis.Another 20 healthy volunteers in Physical Examination Center were enrolled as controls (HC group) .Flow cytometry was used to investigate the characteristics of Tfc in peripheral blood and its correlation with total B lymphocytes, juvenile B lymphocytes, memory B lymphocytes, plasma cells, and liver function parameters.The Kruskal-Wallis H test was used for comparison of continuous data between multiple groups, and the Mann-Whitney U test was used for further comparison between any two groups;Spearman rank correlation was used for correlation analysis.Results The AR group had a significantly higher frequency of Tfc in peripheral blood than the NAR group (U=60, P=0.008) and the HC group (U=2, P<0.001) , and the NAR group had a significantly higher frequency of Tfc than the HC group (U=40, P<0.001) .The frequency of Tfc was positively correlated with total B lymphocytes and juvenile B lymphocytes (r=0.665 and 0.462, both P<0.001) , negatively correlated with memory B lymphocytes and plasma cells (r=-0.453, P=0.001) , and positively correlated with serum gamma-glutamyl transpeptidase level (r=0.371, P=0.026) .Conclusion There is an increase in the frequency of peripheral Tfc in patients who experience acute rejection after liver transplantation, which is correlated with B lymphocytes and hepatic bile duct injury.Tfc may be involved in acute rejection after liver transplantation.

     

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