中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 35 Issue 4
Apr.  2019
Turn off MathJax
Article Contents

Influence of low-level lead exposure on liver function and blood lipids in rats with nonalcoholic fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2019.04.026
Research funding:

 

  • Received Date: 2019-01-07
  • Published Date: 2019-04-20
  • Objective To investigate the influence of low-lead high-fat diet on liver function and blood lipids in rats with nonalcoholic fatty liver disease ( NAFLD) , as well as the expression of sterol regulatory element-binding protein-1 c ( SREBP-1 c) , fatty acid synthase ( FAS) , and acetyl-CoA carboxylase α ( ACCα) in the liver from the aspect of the fatty acid synthesis pathway. Methods A total of 40 clean male Sprague-Dawley rats were given adaptive feeding for 14 days and were then randomly divided into normal group, low-lead exposure group, high-fat diet group, and low-lead high-fat diet group, with 10 rats in each group. The rats were sacrificed after 8 weeks of feeding with the corresponding diet, and serum and liver samples were collected to measure blood lead, liver function, blood lipids, and liver pathology. Western blot and RT-PCR were used to measure the protein and mRNA expression of SREBP-1 c, FAS, and ACCα involved in fatty acid synthesis. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results There were marked liver pathological changes after exposure. The sections from the normal group and the low-lead exposure group had a smooth surface and evenly distributed nuclei, without marked adipocyte infiltration or inflammatory response; the sections from the low-lead high-fat diet group and the high-fat diet group had marked fatty infiltration and inflammatory cell infiltration, and those from the low-lead high-fat diet group had larger fat vacuoles. There were significant differences in blood lead and liver lead between groups ( F = 37. 792 and 21. 458, both P < 0. 001) , and the low-lead exposure group and the low-lead high-fat diet group had significantly higher levels than the normal group ( all P < 0. 05) . There were significant differences in aspartate aminotransferase ( AST) , alanine aminotransferase ( ALT) , high-density lipoprotein cholesterol ( HDL-C) , and low-density lipoprotein cholesterol ( LDL-C) between groups ( F = 35. 791, 24. 422, 37. 287, and 42. 371, all P <0. 001) , and the low-lead exposure group, the high-fat diet group, and the low-lead high-fat diet group had significantly higher AST, ALT, LDL-C, and HDL-C than the normal group ( all P < 0. 05) . Western blot and RT-PCR showed consistent protein and mRNA expression features of SREBP-1 c, FAS, and ACCα, and there were significant differences in the protein and mRNA expression of REBP-1 c, FAS, and ACCα between groups ( protein expression: F = 21. 864, 22. 358, and 57. 761, all P < 0. 001; mRNA expression: F =34. 652, 22. 964, and 42. 384, all P < 0. 001) . Compared with the normal group and the low-lead exposure group, the high-fat diet group and the low-lead high-fat diet group had significant increases in the protein and mRNA expression of SREBP-1 c, FAS, and ACCαin the liver ( all P < 0. 05) . Conclusion Low-lead exposure can aggravate liver function and dyslipidemia in NAFLD rats, possibly by regulating the expression of SREBP-1 c, FAS, and ACCα.

     

  • loading
  • [1]COWAN V, BLAKLEY B.Acute lead poisoning in western Canadian cattle-A 16-year retrospective study of diagnostic case records[J].Can Vet J, 2016, 57 (4) :421-426.
    [2]INDU TH, RAJA D, PONNUSANKAR S.Toxicoepidemiology of acute poisoning cases in a secondary care hospital in rural South India:A 5-year analysis[J].J Postgrad Med, 2016, 62 (1) :48-49.
    [3] LIANG YY.Pueraria, Licorice, Experimental study on the repair of acute liver injury induced by lead poisoning[D].Nanning:Guangxi University of Chinese Medicine, 2015. (in Chinese) 梁云燕.葛根甘草合剂对铅中毒所致大鼠急性肝损害的保护作用[D].南宁:广西中医药大学, 2015.
    [4]LEE CM, TERRIZZI AR, BOZZINI C, et al.Chronic lead poisoning magnifies bone detrimental effects in an ovariectomized rat model of postmenopausal osteoporosis[J].Exp Toxicol Pathol, 2016, 68 (1) :47-53.
    [5]GHANE T, ZAMANI N, HASSANIANMOGHADDAM H, et al.Lead poisoning outbreak among opium users in the Islamic Republic of Iran, 2016-2017[J].Bull World Health Organ, 2018, 96 (3) :165-172.
    [6]MILLS C, ADDERLEY WP.Occupational exposure to heavy metals poisoning:Scottish lead mining[J].Soc History Med, 2017, 30 (3) :hkw084.
    [7]QIU W, CHEN Y, HU X, et al.Analysis on current situation and influencing factors of lead poisoning in children and adolescents in certain region of Yunnan[J].J Kunming Med Univ, 2016, 37 (11) :18-22. (in Chinese) 邱炜, 陈莹, 胡秀, 等.云南某地区儿童及青少年铅中毒现状及影响因素分析[J].昆明医科大学学报, 2016, 37 (11) :18-22.
    [8]GUO Y, WU XY, XIONG W, et al.Prevention and treatment of lead poisoning in children-A review with recent updates[J].Chin J Child Health Care, 2017, 25 (4) :378-381. (in Chinese) 郭妍, 吴晓燕, 熊伟, 等.儿童铅中毒及防治的回顾与新进展[J].中国儿童保健杂志, 2017, 25 (4) :378-381.
    [9]CHEN YQ, LIN SQ, ZHANG C, et al.Three cases of lead poisoning in children caused by"Oral Drugs"[J].Chin J Hyg Rescue, 2016, 2 (2) :131-132. (in Chinese) 陈育全, 林毓嫱, 张程, 等.“嘴药”致儿童铅中毒三例[J].中华卫生应急电子杂志, 2016, 2 (2) :131-132.
    [10]DVM M, OLIVEIRA P, BRANDÄO R, et al.Lead poisoning due to lead-pellet ingestion in griffon vultures (Gyps fulvus) from the Iberian Peninsula[J].J Avian Med Surg, 2016, 30 (3) :274-279.
    [11]MIRACLE V.Lead poisoning in children and adults[J].Dimensions of Critical Care Nursing Dccn, 2017, 36 (1) :71-73.
    [12]ZHANG XL.Feasibility of alanine aminotransferase/aspartate aminotransferase ratio in predicting hepatic steatosis in chronic hepatitis C patients[J].J Clin Hepatol, 2017, 33 (6) :1096-1100. (in Chinese) 张秀丽.ALT与AST比值预测慢性丙型肝炎患者肝脂肪变性的可行性分析[J].临床肝胆病杂志, 2017, 33 (6) :1096-1100.
    [13]RINELLA ME, SANYAL AJ.Management of NAFLD:A stage-based approach[J].Nat Rev Gastroenterol Hepatol, 2016, 13 (4) :196-205.
    [14]XU T, ZHOU J, ZHU J, et al.Carnosic acid protects non-alcoholic fatty liver-induced dopaminergic neuron injury in rats[J].Metab Brain Dis, 2016, 32 (2) :1-9.
    [15]ZHANG Y, MENG T, ZUO L, et al.Xyloketal B attenuates fatty acid-induced lipid accumulation via the SREBP-1c Pathway in NAFLD models[J].Mar Drugs, 2017, 15 (6) :e163.
    [16]ZHAO WX, YAN L, SHAO MY, et al.Effect of Huatan Qu Shi Huoxue prescription on the ADPN/AMPK/ACC signaling pathway in rats with nonalcoholic steatohepatitis[J].J Clin Hepatol, 2017, 33 (5) :932-936. (in Chinese) 赵文霞, 闫乐, 邵明义, 等.化痰祛湿活血方对非酒精性脂肪性肝炎大鼠ADPN/AMPK/ACC通路的影响[J].临床肝胆病杂志, 2017, 33 (5) :932-936.
    [17]YUAN RS, LI H, SUN JH, et al.Lipid-lowering effect and antioxidant activity of polysaccharide from Schisandra Chinensis in rats with non-alcoholic fatty liver disease induced by high-fat diet[J].J Jilin Univ:Med Edit, 2017, 43 (6) :1103-1108. (in Chinese) 苑荣爽, 李贺, 孙靖辉, 等.北五味子多糖对高脂诱导非酒精性脂肪性肝病大鼠的降血脂作用及其抗氧化活性[J].吉林大学学报:医学版, 2017, 43 (6) :1103-1108.
    [18]SHI D, ZHAN X, YU X, et al.Inhibiting CB1 receptors improves lipogenesis in an in vitro non-alcoholic fatty liver disease model[J].Lipids Health Dis, 2014, 13:173.
    [19]ESPE M, RATHORE R, DU Z, et al.Methionine limitation results in increased hepatic FAS activity, higher liver 18∶1 to 18∶0 fatty acid ratio and hepatic TAG accumulation in Atlantic salmon, Salmo salar[J].Amino Acids, 2010, 39 (2) :449-460.
    [20]CHEN X, ZHANG C, ZHAO M, et al.Melatonin alleviates lipopolysaccharide-induced hepatic SREBP-1c activation and lipid accumulation in mice[J].J Pineal Res, 2011, 51 (4) :416-425.
    [21]LI J.Research of repairmen mechanism of curcumin on leadinduced ratsPHhepatotoxicity[D].Shanghai:East China University of Science, 2016. (in Chinese) 黎俊.姜黄素对铅中毒大鼠肝损伤修复作用机制研究[D].上海:华东理工大学, 2016.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1424) PDF downloads(317) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return