Effect of macrophages on the differentiation of mouse induced pluripotent stem cells into hepatic progenitor cells

Tiantian GONG; Shaogang HUANG; Ruizhen SUN; Jingling SHEN; Qiuming LI; Lei LEI; Zhiyan SHAN

doi:10.3969/j.issn.1001-5256.2021.04.025

Volume 37 Issue 4

Apr. 2021

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Article Navigation > Journal of Clinical Hepatology > 2021 > 37(4): 852-856

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Effect of macrophages on the differentiation of mouse induced pluripotent stem cells into hepatic progenitor cells

DOI: 10.3969/j.issn.1001-5256.2021.04.025

1.
Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China
2.
Department of Histology and Embryology, Harbin Medical University (Daqing), Daqing, Heilongjiang 163319, China

Received Date: 2020-09-07
Accepted Date: 2020-10-26
Published Date: 2021-04-20

Abstract

Abstract

Objective To investigate the effect of macrophages (MCs) on the differentiation of mouse induced pluripotent stem cells (iPSCs) into hepatic progenitor cells (HPCs). Methods A total of 24 C57BL/6N mice were used to obtain MCs by peritoneal irrigation, and the supernatant was collected to obtain the conditioned medium of MCs (MC-CDM). Activin A, bone morphogenetic protein 4, and fibroblast growth factor were used to induce the differentiation of mouse iPSCs into HPCs. The differentiation of HPCs were randomly divided into control group (normal medium) and experimental group (MC group; use of MC-CDM medium on day 5 of induction). Morphology, immunofluorescence assay, and Western blot were used to compare the morphology of HPCs and the expression of related proteins between the control group and the MC group. The t-test was used for comparison of continuous data between two groups. Results HPCs derived from iPSCs were established in vitro, and HPCs had the potential to differentiate into hepatocytes. Immunofluorescence assay showed that compared with the D12 control group, the D12 MC group had a significant increase in the protein expression of the HPC-specific protein CK19 (0.901±0.072 vs 0.686±0.097, t=-3.093, P < 0.05). Western blot showed that compared with the D12 control group, the D12 MC group had a significant increase in the protein expression of the HPC-related protein CK19 (1.922±0.103 vs 1.448±0.012, t =-7.881, P < 0.05), as well as a significant increase in the protein expression of the autophagy-related protein LC3 (1.392±0.042 vs 1.101±0.048, t =-5.978, P < 0.05). Conclusion MCs can promote the differentiation of mouse iPSCs into HPCs, possibly by increasing the autophagy level of HPCs.
- Multipotent Stem Cells,
- Hepatic Progenitor Cells,
- Macrophages,
- Cell Differentiation,
- Mice, Inbred C57BL

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Effect of macrophages on the differentiation of mouse induced pluripotent stem cells into hepatic progenitor cells

DOI: 10.3969/j.issn.1001-5256.2021.04.025

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Effect of macrophages on the differentiation of mouse induced pluripotent stem cells into hepatic progenitor cells

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