中文｜English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 37 Issue 5
May  2021
Turn off MathJax
Article Contents
Article Navigation >  Journal of Clinical Hepatology  > 2021  >  37(5): 1075-1080

Clinical significance of serum protoporphyrin Ⅸ measurement in patients with HBV-related chronic liver diseases

DOI: 10.3969/j.issn.1001-5256.2021.05.020
  • 1.

    Department of Rehabilitation Medicine, The Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China

  • 2.

    Class 1625 of School of Clinical Medicine, Xi'an Medical University, Xi'an 710068, China

  • 3.

    Department of Geriatrics, Xianyang Central Hospital, Xianyang, Shaanxi 712000, China

  • 4.

    Department of Gastroenterology, The First Afftliated Hospital of Xi'an Medical University, Xi'an 710077, China

  • Received Date: 2020-12-06
  • Accepted Date: 2021-01-26
  • Published Date: 2021-05-20
    Abstract
  •   Objective  To investigate the value of serum protoporphyrin Ⅸ (PPIX) measurement in evaluating liver damage in patients with HBV-related chronic liver diseases.  Methods  A total of 110 patients who were diagnosed with HBV-related chronic liver diseases in The First Affiliated Hospital of Xi'an Medical University from October 2018 to October 2019 were enrolled as case group [chronic hepatitis B (CHB) group with 50 patients, liver cirrhosis (LC) group with 40 patients, and hepatocellular carcinoma (HCC) group with 20 patients], and 40 healthy individuals were enrolled as control group. High-performance liquid chromatography was used to measure serum PPIX. A one-way analysis of variance was used for comparison of normally distributed continuous data between groups, and the Mann-Whitney U test or the Kruskal-Wallis H test was used for comparison of non-normally distributed continuous data between groups; the chi-square test was used for comparison of categorical data between groups; the receiver operating characteristic (ROC) curve was plotted to analyze diagnostic value; a Spearman correlation analysis was also performed to investigate correlation.  Results  The CHB, HC, and HCC groups had a significantly higher level of PPIX than the control group [44.29 (25.99-85.36) ng/dl, 72.73 (48.28-90.43) ng/dl, and 91.79 (68.34-121.52) ng/dl vs 15.43 (10.87-20.16) ng/dl, all P < 0.05], and the patients with decompensated LC had a significant increase in the level of PPIX compared with those with compensated LC [54.50(29.14~85.65) vs 76.09(53.47~104.37), Z=-2.176, P < 0.05]. PPIX had a sensitivity of > 85% and a specificity of > 60% in the diagnosis of CHB, LC, and HCC. The patients in the latent stage of CHB had a significantly higher level of PPIX than those in the control group (Z=-4.303, P < 0.05). In the patients with LC, PPIX was moderately positively correlated with total bilirubin (TBil) (rs=0.587, P < 0.05) and moderately negatively correlated with albumin (rs=-0.408, P < 0.05); in the patients with HCC, PPIX was moderately positively correlated with TBil (rs=0.470, P < 0.05) and moderately negatively correlated with cholinesterase (rs=-0.459, P < 0.05).  Conclusion  Elevated serum PPIX is an early event of liver damage in patients with HBV-related chronic liver diseases and can thus be used as a sensitive parameter for the early warning and assessment of liver damage.

     

    • FullText(HTML)
  • loading
    • References(18)
  • [1]
    Chinese Society of Infectious Diseases and Chinese Society of Hepatology, Chinese Medical Association. Guidelines for the prevention and treatment of chronic hepatitis B (version 2019)[J]. J Clinl Hepatol, 2019, 35(12): 2648-2669. DOI: 10.3969/j.issn.1001－5256.2019.12.007.

    中华医学会感染病学分会, 中华医学会肝病学分会. 慢性乙型肝炎防治指南(2019年版)[J]. 临床肝胆病杂志, 2019, 35(12): 2648-2669. DOI: 10.3969/j.issn.1001－5256.2019.12.007.
    [2]
    FUJIWARA T, HARIGAE H. Biology of heme in mammalian erythroid cells and related disorders[J]. Biomed Res Int, 2015, 2015: 278536. DOI: 10.1155/2015/278536.
    [3]
    Chinese Society of Hepatology and Chinese Society of Infectious Diseases, Chinese Medical Association. The guideline of prevention and treatment for chronic hepatitis B: A 2015 update[J]. J Clin Hepatol, 2015, 31(12): 1941-1960. DOI: 10.3969/j.issn.1001－5256.2015.12.002.

    中华医学会肝病学分会, 中华医学会感染病学分会. 慢性乙型肝炎防治指南(2015年更新版)[J]. 临床肝胆病杂志, 2015, 31(12): 1941-1960. DOI: 10.3969/j.issn.1001－5256.2015.12.002.
    [4]
    MANSOURI A, GATTOLLIAT CH, ASSELAH T. Mitochondrial dysfunction and signaling in chronic liver diseases[J]. Gastroenterology, 2018, 155(3): 629-647. DOI: 10.1053/j.gastro. 2018.06.083
    [5]
    SACHAR M, ANDERSON KE, MA X. Protoporphyrin Ⅸ: The good, the bad, and the ugly[J]. J Pharmacol Exp Ther, 2016, 356(2): 267-275. DOI: 10.1124/jpet.115.228130.
    [6]
    ZHU FC, BI HQ, ZHAO Y, et al. How hepatitis B virus core protein triggers abnormal metabolism of porphyrin in human liver cancer cells[J]. Chin J Virol, 2019, 35(4): 592-598. DOI: 10.13242/j.cnki.bingduxuebao.003577.

    朱芳成, 毕华强, 赵毅, 等. 乙型肝炎病毒核心蛋白触发人肝癌细胞异常卟啉代谢的机制[J]. 病毒学报, 2019, 35(4): 592-598. DOI: 10.13242/j.cnki.bingduxuebao.003577.
    [7]
    LIU X, LI SH, GAO SL, et al. Preliminary study on changes of hematoporphyrin in patients with chronic liver disease[J]. J Harbin Med Univ, 1992, 26(2): 99-101. https://www.cnki.com.cn/Article/CJFDTOTAL-HYDX199202008.htm

    刘桪, 李少华, 高善玲, 等. 慢性肝病患者血卟啉变化初探[J]. 哈尔滨医科大学学报, 1992, 26(2): 99-101. https://www.cnki.com.cn/Article/CJFDTOTAL-HYDX199202008.htm
    [8]
    GIBSON PR, GRANT J, CRONIN V, et al. Effect of hepatobiliary disease, chronic hepatitis C and hepatitis B virus infections and interferon-alpha on porphyrin profiles in plasma, urine and faeces[J]. J Gastroenterol Hepatol, 2000, 15(2): 192-201. DOI: 10.1046/j.1440-1746.2000.02065.x.
    [9]
    GAO SL, GUO HC, YIN JM, et al. Clinical significance of changes of hematoporphyrin in primary liver cancer[J]. J Harbin Med Univ, 1997, 31(2): 142-144. https://www.cnki.com.cn/Article/CJFDTOTAL-HYDX702.018.htm

    高善玲, 郭惠春, 殷积美, 等. 原发性肝癌血卟啉变化的临床意义[J]. 哈尔滨医科大学学报, 1997, 31(2): 142-144. https://www.cnki.com.cn/Article/CJFDTOTAL-HYDX702.018.htm
    [10]
    NAKANO T, MORIYA K, KOIKE K, et al. Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells[J]. PLoS One, 2018, 13(6): e0198345. DOI: 10.1371/journal.pone.0198345.
    [11]
    YANG Z, PHILIPS JD, DOTY RT, et al. Kinetics and specificity of feline leukemia virus subgroup C receptor (FLVCR) export function and its dependence on hemopexin[J]. J Biol Chem, 2010, 285(37): 28874-28882. DOI: 10.1074/jbc.M110.119131.
    [12]
    KHAN AA, QUIGLEY JG. Control of intracellular heme levels: heme transporters and heme oxygenases[J]. Biochim Biophys Acta, 2011, 1813(5): 668-682. DOI: 10.1016/j.bbamcr.2011.01.008.
    [13]
    KRISHNAMURTHY PC, DU G, FUKUDA Y, et al. Identification of a mammalian mitochondrial porphyrin transporter[J]. Nature, 2006, 443(7111): 586-589. DOI: 10.1038/nature05125.
    [14]
    GAO WY, LI D, CAI DE, et al. Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore[J]. Oncol Rep, 2017, 37(1): 48-56. DOI: 10.3892/or.2016.5225.
    [15]
    GRAZIANI M, SARTI P, ARESE M, et al. Cardiovascular mitochondrial dysfunction induced by cocaine: Biomarkers and possible beneficial effects of modulators of oxidative stress[J]. Oxid Med Cell Longev, 2017, 2017: 3034245. DOI: 10.1155/2017/3034245.
    [16]
    KRIVOSHEEV AB, KONDRATOVA MA, KRIVOSHEEVA TA, et al. The porphyrin metabolism in liver cirrhosis[J]. Ter Arkh, 2013, 85(1): 48-55. http://www.ncbi.nlm.nih.gov/pubmed/23536946
    [17]
    ESMAT S, ELGENDY D, ALI M, et al. Prevalence of photosensitivity in chronic hepatitis C virus patients and its relation to serum and urinary porphyrins[J]. Liver Int, 2014, 34(7): 1033-1039. DOI: 10.1111/liv.12513.
    [18]
    WANG RM, ZHU YF, HE YF, et al. Structures and functions of metalloporphyrin protein conjugates[J]. Prog Chem, 2010, 22(10): 1952-1963. https://www.cnki.com.cn/Article/CJFDTOTAL-HXJZ201010009.htm

    王荣民, 朱永峰, 何玉凤, 等. 金属卟啉蛋白质结合体的结构与功能[J]. 化学进展, 2010, 22(10): 1952-1963. https://www.cnki.com.cn/Article/CJFDTOTAL-HXJZ201010009.htm
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (361) PDF downloads(30) Cited by()
    Proportional views
    Related
        

    The first journal specializing in hepatobiliary and pancreatic diseases in China

    Supervisor：Ministry of Education of the People's Republic of China

    Sponsor：Jilin University

    Academic Support: Chinese Society of Hepatology，Chinese Medical Association

    Address: 461 Xinjiang Road, Changchun

    Submit：0431-88782044

    Peer review：0431-88783542

    Email：lcgdb@vip.163.com

    About Journal

    Submission Guideline

    Journal Online

    Hepatobiliary College

    Guidelines

    YesterdayIP[]YesterdayPV[]TodayIP[]TodayPV[]Online[]

    Website Design © 2020 Editorial Board of Journal of Clinical Hepatology 吉ICP备10000617号-1 Supported by: Beijing Renhe Information Technology Co. Ltd  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return