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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 37 Issue 6
Jun.  2021
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Article Navigation >  Journal of Clinical Hepatology  > 2021  >  37(6): 1373-1378

Protective effect of resolvin D1 pretreatment in a rat model of hepatic ischemia-reperfusion injury and its mechanism

DOI: 10.3969/j.issn.1001-5256.2021.06.030
  • 1.

    Department of Gastroenterology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, China

  • 2.

    School of Clinical Medicine, Hebei University, Baoding, Hebei 071000, China

  • 3.

    Department of Internal Medicine, Yixian People's Hospital, Baoding, Hebei 074200, China

  • 4.

    Department of Internal Medicine, Rongcheng Hospital of Traditional Chinese Medicine, Baoding, Hebei 071700, China

  • 5.

    Department of Internal Medicine, Baoding First Hospital of Traditional Chinese Medicine, Baoding, Hebei 071000, China

  • Received Date: 2020-12-10
  • Accepted Date: 2021-02-01
  • Published Date: 2021-06-20
    Abstract
  •   Objective  To investigate the protective effect of resolvin D1 (RvD1) in a rat model of hepatic ischemia/reperfusion (IR) injury and its association with heme oxygenase-1 (HO-1).  Methods  A total of 36 Sprague-Dawley rats were randomly divided into sham-operation (sham)+phosphate-buffered saline (PBS) group, sham+high-dose RvD1 (10 μg/kg) group, IR+PBS group, IR+low-dose RvD1 (2 μg/kg) group, IR+middle-dose RvD1 (5 μg/kg) group, and IR+high-dose RvD1 (10 μg/kg) group, with 6 rats in each group. RvD1 were intraperitoneally injected at 1 hour before ischemia. A biochemical analyzer was used to measure the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST); ELISA was used to measure the plasma levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8); HE staining was used to observe the histological changes of the liver; Western blot was used to measure the change in HO-1 in liver tissue. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups.  Results  Compared with the IR+PBS group, the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group had significant reductions in the levels of ALT, AST, and the inflammatory factors TNF-α, IL-6, and IL-8 (all P < 0.05), and there were no significant differences between the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group (all P > 0.05). Western blot showed that compared with the IR+PBS group, the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group had a significant increase in the protein expression of HO-1 in the liver (P < 0.05). HE staining showed that compared with the IR+PBS group, the IR+middle-dose RvD1 group and the IR+high-dose RvD1 group had cell swelling and disordered hepatic cords, without massive necrosis.  Conclusion  RvD1 exerts a protective effect against hepatic IR injury in rats by increasing the expression of HO-1 and reducing the levels of inflammatory factors (TNF-α, IL-6, and IL-8) and aminotransferases (ALT and AST).

     

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