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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 36 Issue 12
Dec.  2020
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Article Contents

Value of peripheral blood long non-coding RNA-LET in the diagnosis of chronic hepatitis B cirrhosis

DOI: 10.3969/j.issn.1001-5256.2020.12.014
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  • Received Date: 2020-06-07
  • Published Date: 2020-12-20
  • Objective To investigate the value of peripheral blood long non-coding RNA-LET( lncRNA-LET) in the diagnosis of chronic hepatitis B( CHB) cirrhosis,and to provide a basis for early clinical diagnosis and treatment of liver cirrhosis. Methods A total of 175 CHB patients who attended The Affiliated Hospital of Chengde Medical University from March 2017 to May 2019 were enrolled,among whom52 patients with hepatitis B cirrhosis were enrolled as cirrhosis group and 123 patients without the pathological changes of liver cirrhosis were enrolled as non-cirrhosis group. A total of 40 healthy individuals who underwent physical examination in our hospital during the same period of time were enrolled as normal control group. Liver function parameters and the level of lncRNA-LET in peripheral blood were measured for all subjects. The t-test was used for comparison of continuous data between two groups; an analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups. The chi-square test was used for comparison of categorical data between groups,and the Kruskal-Wallis H test was used for comparison of ranked data. A Pearson correlation analysis was performed to investigate correlation. The receiver operating characteristic( ROC) curve was used to investigate the value of peripheral blood lncRNA-LET in predicting liver cirrhosis. Results Compared with the normal control group,the cirrhosis group and the non-cirrhosis group had significantly higher serum levels of the liver function parameters total bilirubin( TBil),total bile acid( TBA),albumin( Alb),and alanine aminotransferase( ALT)( all P < 0. 05) and a significantly lower serum level of cholinesterase( ChE)( P < 0. 05); compared with the non-cirrhosis group,the cirrhosis group had significantly higher serum levels of TBil,TBA,Alb,and ALT( all P < 0. 05) and a significantly lower serum level of ChE( P < 0. 05). Compared with the normal control group,the cirrhosis group and the non-cirrhosis group had significantly lower relative expression of lncRNA-LET in peripheral blood( P < 0. 05),and the cirrhosis group had significantly lower relative expression of lncRNA-LET in peripheral blood than the non-cirrhosis group( P < 0. 05). The relative expression of lncRNA-LET decreased significantly with the increase in liver fibrosis stage( P < 0. 05). In the patients with CHB,the relative expression of lncRNA-LET in peripheral blood was negatively correlated with liver fibrosis stage,TBil,TBA,Alb,and ALT( r =-0. 352,-0. 372,-0. 364,and-0. 410,all P < 0. 001) and was positively correlated with ChE( r = 0. 340,P < 0. 001). The ROC curve was used to analyze the value of peripheral blood lncRNA-LET in predicting liver cirrhosis,and the area under the ROC curve was 0. 934,with an optimal cut-off value of 0. 833,a sensitivity of 84. 57%,and a specificity of 80. 57%. Conclusion The expression level of lncRNA-LET in peripheral blood decreases with the progression of liver fibrosis and has a good value in the diagnosis of CHB cirrhosis,and therefore,it can be used as a potential biological indicator for the diagnosis of liver cirrhosis.

     

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  • [1] PAPATHEODORIDIS GV,MANOLAKOPOULOS S,SU TH,et al. Significance of definitions of relapse after discontinuation of oral antivirals in HBe Ag-negative chronic hepatitis B[J].Hepatology,2018,68(2):415-424.
    [2] ZHOU XC,LI J,LU W,et al. Diagnostic value of endoscopic ultrasonography combined with liver stiffness measurement and liver histopathology for early liver cirrhosis[J]. Chin J Dig Endosc,2019,36(2):113-118.(in Chinese)周学超,李嘉,陆伟,等.内镜超声联合肝硬度与肝脏病理联合评估早期肝硬化的价值[J].中华消化内镜杂志,2019,36(2):113-118.
    [3] LI WY,LI W,LENG Y,et al. Research progress on relationship between long noncoding RNA and diabetic myocardial ischemia/reperfusion injury[J]. China Med Herald,2019,16(22):50-53.(in Chinese)李文远,李维,冷燕,等.lncRNA与糖尿病心肌缺血再灌注关系的研究进展[J].中国医药导报,2019,16(22):50-53.
    [4] SUN Z,WU XY,WU CL. The association between LncRNA HOTAIR and cancer lymph node metastasis and distant metastasis:A meta-analysis[J]. Neoplasma,2018,65(2):178-184.
    [5] Hepatology Branch of Chinese Medical Association,Infectious Diseases Branch of Chinese Medical Association. The guideline of prevention and treatment for chronic hepatitis B(a 2015update)[J]. J Clin Hepatol,2015,31(12):1941-1960.(in Chinese)中华医学会肝病学分会,中华医学会感染病学分会.慢性乙型肝炎防治指南(2015年更新版)[J].临床肝胆病杂志,2015,31(12):1941-1960.
    [6] XIN SJ,ZHANG LX,ZHU CL,et al. Clinical and pathological diagnostic criteria of chronic viral hepatitis[J]. World Chin J Dig,2001,9(6):726-727.(in Chinese)辛绍杰,张玲霞,朱传琳,等.慢性病毒性肝炎临床与病理的诊断标准[J].世界华人消化杂志,2001,9(6):726-727.
    [7] GUO YC,LU LG. Role of monocyte chemoattractant protein 1and its receptor in the progression of liver fibrosis[J]. J Clin Hepatol,2019,35(12):2793-2795.(in Chinese)郭悦承,陆伦根.单核细胞趋化蛋白-1及其受体在肝纤维化进程中的作用[J].临床肝胆病杂志,2019,35(12):2793-2795.
    [8] GANNE-CARRIN,ZIOL M,de LEDINGHEN V,et al. Accuracy of liver stiffness measurement for the diagnosis of cirrhosis in patients with chronic liver diseases[J]. Hepatology,2006,44(6):1511-1517.
    [9] WANG H,HUO X,YANG XR,et al. STAT3-mediated upregulation of lncRNA HOXD-AS1 as a ceRNA facilitates liver cancer metastasis by regulating SOX4[J]. Mol Cancer,2017,16(1):136.
    [10] CHEN SS,CHEN X,JIANG QY,et al. Expression and prognostic significance of Long non-coding RNA CCAT1 in hepatocellular carcinoma[J]. J Med Res,2018,47(9):86-89.(in Chinese)陈思思,陈霞,蒋琼英,等.长链非编码RNA CCAT1在肝细胞肝癌中的表达及预后意义[J].医学研究杂志,2018,47(9):86-89.
    [11] HUANG W,THOMAS B,FLYNN RA,et al. Retraction Note:DDX5 and its associated lncRNA Rmrp modulate TH17 cell effector functions[J]. Nature,2018,562(7725):150.
    [12] WU QL,ZHANG J. Effects of host gene 1 of long-chain non coding RNA nucleolar small molecule RNA on proliferation and invasion of gastric cancer cells[J]. Chin J Gerontol,2019,39(21):5343-5346.(in Chinese)武秋林,张建.长链非编码RNA核仁小分子RNA宿主基因1对胃癌细胞增殖和侵袭的影响[J].中国老年学杂志,2019,39(21):5343-5346.
    [13] FU Z. Advances in relationship between long noncoding RNA and cardiovascular disease[J]. Adv Cardiovasc Dis,2018,39(2):275-278.(in Chinese)付准.长链非编码RNA与心血管疾病发展关系的研究进展[J].心血管病学进展,2018,39(2):275-278.
    [14] ZHUANG J,SHEN L,YANG L,et al. TGFβ1 promotes gemcitabine resistance through regulating the LncRNA-LET/NF90/miR-145 signaling axis in bladder cancer[J]. Theranostics,2017,7(12):3053-3067.
    [15] WANG PL,LIU B,QIN JW,et al. Effects of lncRNA-LET gene transfection on proliferation,apoptosis,invasion and migration of esophageal squamous carcinoma cells[J]. Jiangsu Med J,2015,41(15):1774-1776.(in Chinese)王鹏立,刘彬,秦建伟,等.LncRNA-LET对食管鳞癌细胞增殖、凋亡、侵袭和迁移能力的影响[J].江苏医药,2015,41(15):1774-1776.
    [16] LI Z,LI BS. Research progress of long-chain non-coding RNA in liver fibrosis[J]. J Chin Physician,2019,21(8):1278-1280.(in Chinese)李哲,李秉寿.长链非编码RNA在肝硬化中的研究进展[J].中国医师杂志,2019,21(8):1278-1280.
    [17] WANG LL,TAO QF,YANG Y,et al. Expression of long noncoding RNA-DHC in hepatocellular carcinoma and its clinical significance[J]. J Clin Pathol Res,2019,39(5):939-946.(in Chinese)王李理,陶其飞,杨远,等.LncRNA-DHC在肝癌中的表达及临床意义[J].临床与病理杂志,2019,39(5):939-946.
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