肝细胞癌p16蛋白的表达及DNA含量分析

孟芝兰; 叶大雄; 宋晓华; 杨春明; 张宁

肝细胞癌p16蛋白的表达及DNA含量分析

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2058
- HTML全文浏览量: 19
- PDF下载量: 893
- 被引次数: 0
出版历程
- 出版日期: 2003-03-20

P16 protein expression and DNA quantitative analysis in human hepatocellular carcinoma

摘要

摘要: 探讨肝细胞肝癌p16蛋白的表达情况及DNA含量的定量分析及临床意义。应用免疫组织化学和多功能真彩色图像分析仪检测 60例肝细胞肝癌p16蛋白表达量和DNA含量 ,同时取 2 0例癌旁肝硬化组织作对照。 60例肝细胞肝癌p16蛋白表达量明显低于癌旁肝硬化组织。p16蛋白表达量随病理分级增高而降低。肝细胞肝癌DNA相对倍体均值 (U值 )随病理分级的增高而增高 (P <0 0 5 )。结论 :p16蛋白的表达情况与肝细胞肝癌的发生发展、恶性程度有关 ,肝细胞肝癌DNA含量与病理学特征及临床生物学行为之间有密切的关系。两者相结合可作为临床判断预后的指标
- 肝细胞肝癌 /
- p16蛋白 /
- 免疫组织化学 /
- 图像分析仪
Abstract: To investigate p16 protein expression and DNA quantitative analysis and clinical significance. p16 protein expression was quantitatively determined by immunohistochemistry in 60 cases of hepatocellular carcinoma. DNA quantitative analysis of 60 cases of hepatocellular carcinoma was performed by imagecytometry. Simultaneously 20 cases of cirrhosis adjacent tumor tissues were contrasted. p16 protein expression in 60 cases of hepatocellular carcinoma was significantly lower than that in cirrhosis adjacent tumor tissues. The expression of p16 protein becomes lower with the increase in grading. The mean of DNA relative ploid (Uvalue) in hepatocellular carcinoma was from 1.08 to 3.17. U value becomes higher with the increase in grading (P<0.05) . Abnormal expression of p16 protein is closely correlated to the occurrence, development and malignancy degree of hepatocellular carcinoma. DNA quantity in hepatocellular carcinoma is closely correlated to pathological feature and clinical biological behavior. Combining both may serve as an indicator to predict the prognosis of patients.
- Hepatocellular carcinoma /
- Protein p16 /
- Immunohistochemistry /
- Image cytometry

HTML全文

参考文献(7)

[1]TannapfelA , BusseC , WeinansL , etal.INK4a-ARFalterationsandp53mutationsinhepatocellularcarcinomas[J].Oncogene, 2001, 20 (48) ∶7104-9.

[2]WeihrauchM , BenickeM , LehnertG , etal.FrequentK -ras-2muta tionsandp16 (INK4A) methylationinhepatocellularcarcinomasinwork ersexposedtovinylchloride[J].BrJCancer, 2001, 84 (7) ∶982-9.

[3]MatsudaY , IchidaT , MatsuzawaJ, etal.P16 (INK4) isinactivatedbyextensiveCPGmethylationinhumanhepatocellularcarcinoma[J].Gas troenterology, 1999, 116 (2) ∶394-400.

[4]SerranoM , HannonGJ , BeachD .Anewregulatorymotifincell-cyclecontrolcausingspecificinhibitionofcyclinD/CDK4[J].Nature, 1993, 366∶704-707.

[5]汤钊猷, 余业勤主编.原发性肝癌第二版[M].上海:上海科学技术出版社, 1999.498.

[6] FarandaA , CostaA , CanovaS , etal.ImageandflowcytometricanalysesofDNAcontentinhumansolidtumor.Acomparativestudy[J].AnalQuantCytolHistol, 1997, 19∶338-44.

[7]王鲁平, 虞积濯, 丁华野, 等.胃癌中生长抑素和DNA倍体的相关表达及其预后意义[J].临床与实验病理学杂志, 1998, 14∶23-25.

施引文献

资源附件(0)

访问统计