中国兰州地区丙型肝炎病毒感染的基因型分布特征

张文杰; 刘丽君; 杜绍财; 李朝霞; 卢青云; 杨少华

中国兰州地区丙型肝炎病毒感染的基因型分布特征

1. 甘肃省第二人民医院感染科
2. 北京大学人民医院,北京大学肝病研究所
3. 甘肃省第二人民医院检验科
4. 兰州市铁路局疾病与预防控制所

基金项目:

国家“十五”科技攻关计划资助项目(2001BA705B06、2004BA718B)；甘肃省科学技术厅自然科学基金项目(0710RJZA005)；

详细信息

中图分类号: R512.63
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出版历程
- 出版日期: 2010-03-20

The prevalence of hepatitis C virus genotype in Lanzhou area

Research funding:

摘要

摘要: 目的了解中国兰州地区丙型肝炎病毒(HCV)感染基因型分布特点。方法筛选41例来自兰州的HCVRNA阳性患者血清样品,扩增HCV5′非编码区(5’NCR),得到257bp的聚合酶链反应(PCR)产物,并对这些产物进行测序。然后将所得序列与GenBank参考序列比对,并作同源性分析和进化分析。结果 41例样品中共检出1b型感染25例(61.0%),1c型感染8例(19.5%),2a型4例(9.8%),1a型3例(7.3%),3b型感染1例(2.4%)。结论兰州地区的基因型分布以1b型为主,并首次在中国检出1c型感染,且感染率仅低于1b型;此外还检出2a、1a和3b;未检出4、5和6型等基因型。
- 肝炎病毒 /
- 肝炎,丙型 /
- 基因型 /
- 5’非编码区
Abstract: Objective To determine the prevalence of Hepatitis C virus (HCV) genotype in Lanzhou area. Methods 41 serum samples were collected from HCV patients in Lanzhou area. HCV genotypes were determined by amplification of 5’NCR region using RT-PCR followed by direct sequence study. The result was further analyzed by homologous and phylogenetic analysis using the complete HCV genome sequence from Gene Bank as a reference. Results From the total of 41 HCV RNA positive samples, the prevalence of HCV genotype in the area were 61.0% for 1b, 19.5% for 1c, 9.8% for 2a, 7.3% for 1a and 2.4% for 3b, with the lack of 4-6 genotypes. Conclusion This study indicates that HCV genotype 1b and 1c are the major HCV genotypes in Lanzhou area.
- hepatitis viruses /
- hepatitis C /
- genotype /
- 5’ noncoding region

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参考文献(15)

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