中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 2
Feb.  2016
Turn off MathJax
Article Contents

Expression and roles of poly(ADP-ribose) polymerase-1 and caspase-3 in pancreatic cancer and adjacent tissues

DOI: 10.3969/j.issn.1001-5256.2016.02.029
  • Received Date: 2015-06-04
  • Published Date: 2016-02-20
  • Objective To investigate the protein expression of poly( ADP- ribose) polymerase- 1( PARP- 1) and caspase- 3 in pancreatic cancer and adjacent tissues. Methods A total of 66 cancer tissue samples and 113 samples of adjacent tissues were collected from the patients who underwent surgical treatment with a confirmed diagnosis of pancreatic cancer by postoperative pathological examinations in The First Hospital of Lanzhou University from January 2013 to June 2014,and immunohistochemical examinations were performed to measure the expression of PARP- 1 and caspase- 3 in these samples. The chi- square test was applied for comparison of categorical data between groups. Results Of all the 66 samples of pancreatic cancer,53 had varying degrees of PARP- 1 expression,with a positive rate of80. 3%; of all the 133 samples of adjacent tissues,39 had PARP- 1 expression,with a positive rate of 34. 5%; the expression of PARP-1 in the samples of pancreatic cancer was significantly higher than that in the samples of adjacent tissues( χ2= 34. 79,P < 0. 01). The expression of PARP- 1 in moderately and poorly differentiated samples of pancreatic cancer( 18 /25,72%; 10 /14,71. 4%) was significantly higher than that in highly differentiated samples( 3 /14,21. 4%)( χ2= 10. 76,P < 0. 01). Of all the 66 samples of pancreatic cancer,47 had varying degrees of caspase- 3 expression,with a positive rate of 71. 2%; of all the 133 samples of adjacent tissues,71 had caspase-3 expression,with a positive rate of 62. 8%. The expression of caspase- 3 in highly differentiated samples of pancreatic cancer( 11 /18,61. 1%) was significantly higher than that in moderately and poorly differentiated samples( 4 /20,20%; 0 /9,0)( χ2= 11. 44,P < 0. 01).Conclusion The expression level of PARP- 1 in the samples of pancreatic cancer is significantly higher than that in the samples of adjacent tissues,and the strong positive rate of PARP- 1 increases with the decrease in the degree of differentiation; the expression of caspase- 3 is similar between pancreatic cancer and adjacent tissues,and the strong positive rate of caspase- 3 decreases with the decrease in the degree of differentiation. The expression levels of PARP- 1 and caspase- 3 may be related to the development and progression of pancreatic cancer.

     

  • loading
  • [1] TAN J.Analysis of the lastest incidence and fatality rate of tumors in China and the rest of the world[R].Chin Cancer Registry Annu Rep,2013.(in Chinese)谭健.中国和全球最新肿瘤发病率和死亡率解析[R].中国肿瘤登记年报,2013.
    [2]FENG JJ,JIA GZ,TAN GY,et al.Changes of serum level of carbohydrate antigen CA19-9 in patients with liver diseases and its clinical significance[J].The J Pract Med,2008,25(9):1043-1044.(in Chinese)逢金聚,贾桂芝,谭国英,等.肝病患者肿瘤标记物CA199水平变化及其临床意义[J].实用医学杂志,2008,25(9):1043-1044.
    [3]KIEBACK DG.Adenovirus-mediated thymidine kinase gene therapy induces apoptosis in human epithelial O-varian cancer cells and damages PARP-1[J].In Vivo,2009,23(1):77-80.
    [4]CHO BB,TOLEDO-PEREYRA LH.Caspase-independent programmed cell death following ischemic stroke[J].J Invest Surg,2008,21(3):141-147.
    [5]NOSHO K,YAMAMOTO H,MIKAMI M,et al.Overexpression of poly(ADP-ribose)polymerase-1(PARP-1)in the early stage of colorectal carcinogenesis[J].Eur J Cancer,2006,42(14):2374-2381.
    [6]OSSOVSKAYA V,KOO IC,KALDJIAN EP,et al.Upregulation of poly(ADP-ribose)polymerase-1(PARP-1)in triple-negative breastcancer and other primary human tumor types[J].Genes Cancer,2010,1(8):812-821.
    [7]LI Y,WEI W,LYU SQ,et al.Effects of PARP-1 on angiogenesis of ovarian cancer[J].J Shandong Univ:Health Sciences,2014,52(4):97-106.(in Chinese)李燕,尉蔚,吕树卿,等.PARP-1对卵巢癌血管生成的影响[J].山东大学学报:医学版,2014,52(4):97-106.
    [8]NOMURA F,YAGUCHI M,TOGAWA A,et al.Enhancement of polyadenosine diphosphate ribosylation in human hepatocellular carcinoma[J].Gastroenterol Hepatol,2000,15(7):529-535.
    [9]FREDERICK K,MORITZVON W,ALBRECHT S,et al.High nuclear poly-(ADP-ribose)-polymerase expression is prognostic of improved survival in pancreatic cancer[J].Histopathology,2012,61(6):409-416.
    [10]XIE QX,LIN XC,HAN CX,et al.Significance of Caspase-3 expression in renal cell cancer[J].China Med Eng,2005,13(5):486-488.(in Chinese)谢庆祥,林吓聪,韩聪祥,等.Caspase-3在肾细胞癌中的表达及其意义[J].中国医学工程,2005,13(5):486-488.
    [11]WINTER NR,KRAMER A.Loss of Caspase-1 and Caspase-3 protein expression in human prostate cancer[J].Cancer Res,2001,61(10):1277-1232.
    [12]WANG CY,MENG LX,DING ZJ.Expression of PTEN,Survivin and Caspase-3 in human esophageal squmous carcinoma[J].J Mod Oncol,2015,23(4):493-496.(in Chinese)王传艳,孟令新,丁兆军.PTEN、Survivin和Caspase-3在食管鳞癌中的表达[J].现代肿瘤医学,2015,23(4):493-496.
    [13]LONG H,WU QM,MA SL,et al.Expression of Midkine and Caspase-3 and their relationship with apoptosis in pancreatic cancer[J].World Chin J Dig,2008,16(35):4015-4019.(in Chinese)龙辉,吴清明,马松林,等.Midkine和Caspase-3在胰腺癌中的表达与细胞凋亡的关系[J].世界华人消化杂志,2008,16(35):4015-4019.
    [14]DU Y,FENG YZ,LI F.Expression of livin and caspase-3 and their clinical significance in pancreatic carcinoma[J].Chin J Clin Exp Pathol,2009,25(6):610-614.(in Chinese)杜缘,冯一中,李峰.胰腺癌中livin、caspase-3的表达及其临床意义[J].临床与实验病理学杂志,2009,25(6):610-614.
    [15]KOH DW,DAWSON TM,DAWSON VL.Mediation of cell death by poly(ADP-ribose)polymerase-1[J].Pharmacol Res,2005,52(1):5-14.
    [16]SUI WY,CHEN CY,LUO ZY,et al.The expression of PAPR-1 and Caspase-3 in human breast cancer and their clinical significance[J].J Univ Sourth China:Med Edit,2010,38(2):210-213.(in Chinese)眭文妍,陈春燕,罗招阳,等.PARP-1和Caspase-3在乳腺癌组织中的表达及临床意义[J].南华大学学报:医学版,2010,38(2):210-213.
    [17]GAO J,CHEN HL.Caspase-3,PARP proteins expression and their significances in human lung cancer[J].J Mathematical Med,2006,19(1):34-36.(in Chinese)高俊,陈洪雷.Caspase-3、PARP蛋白在肺癌组织中的表达及临床意义[J].数理医药学杂志,2006,19(1):34-36.
    [18]QI SQ,DE S,LI DM,et al.Expressions of PARP-1,Caspase-3,survivin and Bax in cervical squamous cell carcinoma tissues and their clinical significances[J].J Jilin Univ:Med Edit,2013,39(3):512-516.(in Chinese)齐斯琴,德胜,李冬梅,等.子宫颈鳞癌组织中PARP-1、Caspase-3、survivin和Bax的表达及其临床意义[J].吉林大学学报:医学版,2013,39(3):512-516.
    [19]HU SM,ZHANG SD,HAO YP,et al.Expressions and significance of PARP-1 and Caspase-3 in colorectal neoplasms[J].Chin J Gastroenterol,2009,14(2):83-87.(in Chinese)胡顺明,张世栋,郝艳萍,等.PARP-1和Caspase-3在结直肠肿瘤中的表达和意义[J].胃肠病学,2009,14(2):83-87.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (275) PDF downloads(218) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return