A preliminary study of the role of neutrophil extracellular traps in patients with primary biliary cholangitis

Yiyan OU; Nana CUI; Yao LI; Qiwei QIAN; Xiong MA; Zhengrui YOU; Min LIAN; Qixia WANG

doi:10.3969/j.issn.1001-5256.2022.04.014

Volume 38 Issue 4

Apr. 2022

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Article Navigation > Journal of Clinical Hepatology > 2022 > 38(4): 810-814

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A preliminary study of the role of neutrophil extracellular traps in patients with primary biliary cholangitis

DOI: 10.3969/j.issn.1001-5256.2022.04.014

Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai 200001, China

Research funding:

National Natural Science Foundation of China (82070581);

National Natural Science Foundation of China (81800504);

National Natural Science Foundation of China (81770564);

National Natural Science Foundation of China (81790634);

"Chen Guang" project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (19CG16);

the Municipal Human Resources Development Program for Outstanding Young Talents in Medical and Health Sciences in Shanghai (2017YQ037);

Shanghai Rising-Star Program (18QA1402700)

More Information

Corresponding author: LIAN Min, sophialian24@163.com(ORCID: 0000-0003-2122-1614); WANG Qixia, wqx0221155@126.com(ORCID: 0000-0002-8300-6314)
Received Date: 2021-11-30
Accepted Date: 2022-01-08
Published Date: 2022-04-20

Abstract

Abstract

Objective To investigate the expression level of neutrophil extracellular traps (NET) in the peripheral blood and liver tissue of primary biliary cholangitis (PBC) patients and its correlation with clinical biochemical parameters. Methods A total of 24 PBC patients who were admitted to Renji Hospital, Shanghai Jiao Tong University School of Medicine, from August 2016 to August 2020 were enrolled, as well as 8 patients with primary sclerosing cholangitis (PSC) and 19 patients with autoimmune hepatitis (AIH) matched for age, and 19 healthy individuals were enrolled as healthy control group (HC group). The serum level of myeloperoxidase (MPO) was measured, and its correlation with clinical indices were analyzed. Immunofluorescence assay was used to measure the expression of NET in the liver of PBC patients, and an in vitro experiment was to compare the ability of peripheral blood neutrophils to produce NET between PBC patients and healthy controls. Normally distributed continuous data were expressed as mean±standard deviation, and the independent samples t-test was used for comparison between two groups; for the non-normally distributed continuous data expressed as M(P₂₅-P₇₅), the Kruskal-Wallis H test was used for comparison between multiple groups, and the Mann-Whitney U test was used for comparison between two groups. A correlation analysis was performed for MPO level and liver-related laboratory markers, and the Spearman's correlation coefficient was calculated. Results The serum level of MPO in the PBC group was increased to 811.21 (450.67-1 216.20) ng/mL, which was significantly higher than that in the AIH group [468.58 (142.63-812.43) ng/mL] and the HC group [357.54 (203.52-811.21) ng/mL] (P < 0.05), suggesting that there was a significant increase in the production of NET in peripheral blood of PBC patients. The PSC patients had a serum MPO level of 763.56 (489.59-1 633.14) ng/mL, which was significantly higher than that in the HC group (P < 0.05). MPO level was positively correlated with alkaline phosphatase (r=0.500, P < 0.05), gamma-glutamyl transpeptidase (r=0.426, P < 0.05), alanine aminotransferase (r=0.521, P < 0.05), and aspartate aminotransferase (r=0.547, P < 0.01). Confocal immunofluorescence showed colocalization of H3Cit and MPO in the liver of PBC patients. In vitro experiment showed that compared with the HC group, the PBC group had an increase in NET produced by peripheral blood neutrophils after in vitro stimulation and an increase in spontaneous production of NET. Conclusion There is an increase in NET in peripheral blood and liver of PBC patients, and the content of peripheral blood NET is positively correlated with biochemical parameters of liver function. NET may become a novel biomarker for assessing the severity of PBC.
- Primary Biliary Cholangitis,
- Neutrophils,
- Extracellular Traps

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References

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A preliminary study of the role of neutrophil extracellular traps in patients with primary biliary cholangitis

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A preliminary study of the role of neutrophil extracellular traps in patients with primary biliary cholangitis

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