The experiment aimed to study the protective effect and its mechanism of oxymatrine (OM) on the fulminant hepatic failure (FHF) induced by injecting lipopolysaccharide (LPS) in galactosamine (GalN) sensitized mice.Thirty mice were divided into three groups:OM group (100mgkg -1 d -1 ×3, ip) , model group and normal group.The serum levels of alanine aminotransferase (ALT) and tumor necrosis factor alpha (TNF α) in all mice were determined at 7.5 hours after injecting GaIN/LPS.At the same time, the liver tissues were takan for HE staining and the immunohistochemical detection of Fas and its ligand (FasL) .Results:The serum levels of ALT and TNF α in mice of OM group were decreased significantly than that of model group (P<0.05 and 0.01, respectively) .Both the degrees of liver injury and expression of Fas and FasL in mice of OM group were reduced obviously than that of model group (P<0.01) .Conclution:It is possible that OM blocks the hepatocyte apoptosis and necrosis induced by LPS through inhibiting the activity of TNF α and the expression of Fas and FasL in liver tissue.
DownLoad: