中文English
ISSN 1001-5256
CN 22-1108/R
Issue 2
Feb.  2006
Turn off MathJax
Article Contents

骨髓干细胞在大鼠肝再生环境中的分化

  • Published Date: 2006-02-20
  • To investigate the differentiation of rat bone marrow stem cells in liver regeneration.Bone marrow cells were obtained from the tibia of rat with partial hepatectomy.The bone marrow stem cells were enriched from the bone marrow cells by fluorescence-activated cell sorting.The bone marrow stem cells were labeled with PKH26-GL in vitro and then autotransplanted by portal vein injection.After 2 weeks, the livers were examined by albumin and ck 8 immunohistochemistry.The results show that there were PLH26-GL-labeling cells among hepatocytes in the liver sections, and some of the cells expressed albumin and ck 8.The results suggest that rat bone marrow stem cells can differentiate into hepatocyte in regenerative environment after partial hepatectomy, bone marrow stem cells may participate in liver regeneration after partial hepatectomy.

     

  • loading
  • [1]Theise ND, Badve S, Saxena R, et al.D erivation of hepatocytesfrom bone marrow cells in m ice after rad iation-induced myeloab la-tion[J].Hepatology, 2000, 31 (1) ∶235-240.
    [2]A lisonMR, Pou lsom R, Jeffery R, et al.Hepatocytes from non-he-patic adu lt stem cells[J].Nature, 2000, 406 (6793) ∶257.
    [3]Theise ND, N immakayalu M, Gardner R, et al.L iver from bonemarrow in humans[J].Hepatology, 2000, 32 (1) ∶11-16.
    [4]Petersen BE, Bowen WC, Patrene KD, et al.Bone marrow as a po-tential source of hepatic oval cells[J].Science, 1999, 284 (5417) ∶1168-1170.
    [5]展玉涛, 魏来, 陈红松, 等.用流式细胞仪富集大鼠骨髓干细胞[J].免疫学杂志, 2003, 19 (6) ∶471-473.
    [6]Sell S.Heterogeneity and plasticity of hepatocyte lineage cells[J].Hepatology, 2001, 33 (3) ∶738-50.
    [7]Hendrikx PJ, Martens CM, Hagenbeek A, et al.Hom ing of fluores-cently labeled murine hematopoietic stem cells[J].Exp Hematol, 1996, 24 (2) ∶129-40.
    [8]Oh D J, Lee GM, Francis K, et al.Phototoxicity of the fluoresentm embrane dyes PKH2 and PKH26 on the human hematopoietic kG1 a progen itor cell line[J].Cytom etry, 1999, 36 (4) ∶312-18.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1845) PDF downloads(940) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return