丹黄方对部分肝叶切除大鼠肝组织中Tec的影响

Objective To explore the effect of DanHuangFang (DHF) on the expression of Tec mRNA in the liver regeneration model with partial liver excision in rats.Methods The liver regeneration model with partial liver excision in rat was employed.Twenty four Wistar rats were randomly divided into four groups: the sham operation group, the operation group, the DHF group and the pHGF group.The operation of partial liver excision was carried on in the latter three group rats.From three days before the operation to 48 hours after the operation, the rats in the DHF group were lavaged with DHF (10g/kg) once a day, and intraperitoneally injected with sodium chloride (4.0ml/kg) simultaneously.The rats in the pHGF group were lavaged with sodium chloride (4.0ml/kg) and intraperitoneally injected with pHGF (1ml/100g) .The rats in the sham operation and the operation group were only lavaged and intraperitoneally injected with sodium chloride (4.0ml/kg) .The expression of Tec mRNA was detected by RT-PCR methods.Results The expression of Tec mRNA in DHF group was significantly higher than that of the sham operation and the operation group (P<0.01) .And there was no difference between the DHF group and pHGF group in the expression of HGFmRNA.Conclusion DHF can promote the expression of Tec mRNA in the liver regeneration model with partial liver excision.