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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 8
Aug.  2012
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Influence of tight junction protein claudin-1 on the susceptibility to HCVcc

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  • Published Date: 2012-08-20
  • Objective To study the influence of the tight junction protein, claudin-1, on susceptibility to HCV in cell culture (HCVcc) . Methods An enhanced green fluorescent protein (EGFP eukaryotic expression vector, pEGFP-claudin-1, was constructed and transfected into human embryonic kidney (HEK) -293T cells with lipofectamine.The expression of claudin-1 and corresponding susceptibility to HCVcc was analyzed by fluorescence assay and RT-PCR, respectively.A chemically synthesized small interfering (si) RNA targeting claudin-1 was transfected into Huh-7.5 hepatoma cells and the effect of claudin-1 silencing on susceptibility to HCVcc was tested by Western blotting and quantitative RT-PCR. Results Expression of claudin-1 in HEK-293T cells conferred susceptibility to HCV infection.Claudin-1 expression was reduced in Huh-7.5 cells by targeted siRNA.Susceptibility to HCVcc was markedly reduced in claudin-1-HCV silenced Huh-7.5 cells. Conclusion Tight junction protein claudin-1 expression is an important determinant of a cell's susceptibility to infection in culture and may contribute to virus tropism for particular cell phenotypes.

     

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