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Volume 41 Issue 7
Jul.  2025
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Article Navigation >  Journal of Clinical Hepatology  > 2025  >  41(7): 1351-1357

Therapeutic effects of dental pulp stem cells in a mouse model of autoimmune hepatitis and related immunoregulatory mechanisms

DOI: 10.12449/JCH250719
  • 1.

    Department of Stomatology，Beijing Jishuitan Hospital，Capital Medical University，Beijing 100035，China

  • 2.

    Beijing Engineering Research Center of Immunocellular Therapy，Beijing 102609，China

  • 3.

    Department of Infectious Diseases，Peking University First Hospital，Beijing 100034，China

Research funding:

Beijing Nova Program (20220484166)

More Information
  • Corresponding author: LI Yin， leeyin78@163.com （ORCID： 0009-0008-8606-2769）; WANG Guiqiang， john131212@hotmail.com （ORCID： 0000-0003-0515-7974）
  • Received Date: 2025-05-26
  • Accepted Date: 2025-06-26
  • Published Date: 2025-07-25
    Abstract
  •   Objective  To investigate the therapeutic effect of dental pulp stem cells （DPSCs） on autoimmune hepatitis in invivo and in vitro experiments and the related mechanism.  Methods  An in vitro co-culture system was used to evaluate the immunoregulatory effect of DPSCs， and 32 mice were randomly divided into healthy control group， model group， positive drug group， and DPSCs treatment group， with 8 mice in each group. The serum levels of alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， and inflammatory factors were measured， and HE staining was used to assess liver pathological injury. An analysis of variance was used for comparison of normally distributed continuous data between multiple groups， and the least significant difference t-test was used for further comparison between two groups.  Results  The in vitro experiment showed that the positive rates of CD105， CD73， and CD90 in DPSCs were 99.97%， 100%， and 99.53%， respectively， while the positive rates of CD34， HLA-DR， and CD45 were 0.56%， 0.17%， and 0， respectively. DPSCs significantly inhibited the proliferation of Th1 and Th17 subsets， with inhibition rates of 31.32% and 45.76%， respectively； DPSCs promoted the proliferation of Treg （CD4+CD25+FoxP3+）， with a promoting rate of 52.29%. DPSCs had an inhibition rate of 93.70% on the proliferation of lymphocytes. In the mouse model of autoimmune hepatitis， compared with the model group， the DPSCs treatment group had significant reductions in the serum levels of ALT and AST， with reduction rates of 66.8% and 60.0%， respectively （t=3.321 and 2.907， P=0.007 5 and 0.017 5） and significant reductions in the inflammatory factors tumor necrosis factor-α and interleukin-1β， with reduction rates of 57.5% and 71.3%， respectively （t=2.484 and 2.796， P=0.039 8 and 0.020 6）， and histopathological examination showed no significant improvement in periportal bridging necrosis （t=1.969， P=0.098）.  Conclusion  DPSCs effectively alleviate immune-mediated liver injury through immunoregulation， which provides an experimental basis for clinical translation.

     

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