中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 32 Issue 1
Jan.  2016
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Changes in serum asymmetric dimethylarginine levels in progression of liver inflammation in an experimental rat model of nonalcoholic fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2016.01.029
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  • Received Date: 2015-06-18
  • Published Date: 2016-01-20
  • Objective To investigate the changes in serum asymmetric dimethylarginine( ADMA) levels in the progression of liver inflammation in a rat model of nonalcoholic fatty liver disease( NAFLD). Methods The rat model of NAFLD was established through high- fat cream by gavage and these rats were considered the experimental group( group M),and the rats in the normal control group( group C) were given normal saline by gavage within the same period of time. Liver samples and serum samples were collected from these rats. Serum alanine aminotransferase( ALT) and ADMA levels and the content of nitric oxide( NO) in liver tissue homogenate were measured at 4,8,and 12 weeks for group M( M4,M8,and M12) and 12 weeks for group C( C12),and NAFLD activity score( NAS) was evaluated for both groups at the same time point. As for the data that were normally distributed and had homogeneity of variance,one- way analysis of variance was applied for comparison between multiple groups,and the least significant difference( LSD) t- test was applied for comparison within the same group; as for the data that were normally distributed and had heterogeneity of variance,Kruskal- Wallis H test was applied for comparison between multiple groups. Pearson correlation analysis was applied for correlation analysis. Results Compared with the C12 group,the M12 group had significantly increased serum ADMA and ALT levels and content of NO in liver tissue homogenate( P = 0. 010,P < 0. 001,and P < 0. 001,respectively). Serum ADMA level was not significantly correlated with serum ALT level( r = 0. 195,P = 0. 228),and was positively correlated with the content of NO in liver tissue homogenate and NAS in rats( r = 0. 631,P < 0. 001; r = 0. 782,P < 0. 001).Conclusion In rats with NAFLD,serum ADMA level gradually increases with the progression of liver inflammation,and is positively correlated with the content of NO and NAS. The role of ADMA as a new inflammatory factor for evaluating the grade of chronic liver inflammation in NAFLD needs to be further investigated.

     

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