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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 11
Nov.  2018
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Effect of plasma exchange on bioactivity of peripheral blood CD34+ cells in patients with acute-on-chronic (subacute) liver failure

DOI: 10.3969/j.issn.1001-5256.2018.11.023
  • Published Date: 2018-11-20
  • Objective To investigate the effect of improvement in internal environment after plasma exchange on the bioactivity of peripheral blood CD34+cells in patients with acute-on-chronic ( subacute) liver failure. Methods The peripheral blood CD34+cells from patients with acute-on-chronic ( subacute) liver failure were cultured in vitro using the medium containing with the serum collected before or after plasma exchange. The growth curves of the two groups of cells were recorded and compared. RT-PCR was used to detect the expression of the cell surface markers PK-M2, Integrin-β1, and L-PK, and immunofluorescence was used to detect the expression of Integrin-β1. A repeated measures analysis of variance was used for comparison of continuous data between groups. Results The CD34+cells in the medium containing the serum collected after plasma exchange grew better than those in the medium containing the serum collected before plasma exchange ( P < 0. 05) . PK-M2 and Integrin-β1 were detected in the CD34+cells of both groups, but L-PK was not detected in either group. Conclusion For patients with liver failure, the improvement in internal environment after plasma exchange helps to maintain the bioactivity of peripheral blood CD34+cells, thus improving the efficacy of stem cell transplantation for liver failure.

     

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