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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 36 Issue 3
Mar.  2020
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Regulatory effect of water extract of Jianpi Xiaoji prescription on autophagy of human liver cancer SMMC7721 cells

DOI: 10.3969/j.issn.1001-5256.2020.03.023
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  • Received Date: 2019-12-06
  • Published Date: 2020-03-20
  • Objective To investigate the anti-liver cancer mechanism of water extract of Jianpi Xiaoji prescription and its effect on cell autophagy flow and autophagy-related proteins. Methods SMMC7721 cells were treated with water extract of Jianpi Xiaoji prescription.CCK-8 assay was used to measure cell proliferation; flow cytometry was used to measure cell apoptosis rate; Western blot was used to measure the expression of the autophagy-related proteins Beclin1,LC3,and P62; the formation of autophagosomes and the fusion of autophagosomes and lysosomes were monitored. The independent samples t-test was used for comparison of continuous data between two groups; a one-way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results The CCK-8 assay showed that cell proliferation was significantly inhibited at 24,48,72,and 96 hours after the drug was added,and there was a significant difference between the drug treatment group and the control group( t =28. 458,81. 093,85. 328,and 100. 158,all P < 0. 001). Flow cytometry showed that compared with the control group,the drug treatment group had a significant increase in the apoptosis of liver cancer SMMC7721 cells( t =-42. 629,P < 0. 001). Western Blot showed that compared with the control group,the drug treatment group had significantly downregulated expression of Beclin1 and significantly upregulated expression of the autophagy markers LC3-Ⅱ and P62. The flow of autophagosomes to autolysosomes was blocked in the drug treatment group,and there was a significant difference between the drug treatment group and the control group( F = 31. 155,P < 0. 001). Conclusion The water extract of Jianpi Xiaoji prescription can inhibit the proliferation of human liver cancer SMMC-7721 cells and promote apoptosis; meanwhile,it can upregulate the expression of Beclin1,downregulate the expression of LC3 and P62,and block the formation of autophagy flux.

     

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