肝组织中Chymase浓度在自身免疫性肝病肝纤维化中的意义

To investigate spatial relatioship betwwen human chymase and fibrosis in liver specimens affected by autoimmune hepatitis (AIH) or primary biliary cirrhosis (PBC) .The chymase levels were determined by using the method of enzyme linked immunosorbant assay (ELISA) in 15 cases of AIH and 14 cases of PBC.11 cases of acute hepatitis (AH) were used as control. Mast cell distribution in the specimens was determined immunohistochemically using anti-chymase antibody. The mean amounts of chymase in livers with AIH and PBC were (15.53±4.89) ng/mg and (12.67±3.69) ng/mg respectivly.Hepatic chymase in AIH and PBC was significantly more abundant than in AH (2.72±1.02) ng/mg, P<0.01.When sections from patients with AIH and PBC were immunostained for chymase, immunoreactive mast cells were detected in portal areas and sinusoidal walls, coinciding with zones of fibrosis. Chymase appears to be involved in hepatic fibrosis in AIH and PBC.