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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 1
Jan.  2007
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人胎肝干细胞体外分离、培养及鉴定

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  • Published Date: 2007-01-20
  • To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics.The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition.The primary cell propagated 8 times and were small fusiform or triangle and smaller than hepatocytes with larger nucleus and less plasma;it was 88.2% in the growing period of 4th generation cells and the stains of ALB and CK19 were positive in Immunocytochemical.The liver stem cells exist in the human fetal liver and maybe become the good cell resources for cell transplantation and liver transplantation.

     

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      沈阳化工大学材料科学与工程学院 沈阳 110142

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