熊去氧胆酸对大鼠免疫性肝纤维化的干预作用研究

To observe the effect of ursodeoxycholic acid (UDCA) on fiber deposition and expression of PDGF-B, TIMP-1 in porcine serum-induced rat liver fibrosis, and to study the mechanism.64 rats were divided into model group, drug therapy group, drug contrast group and normal contrast group (16 rats in every group) .Porcine serum was injected into rat abdominal cavity to induce hepatic fibrosis model.At the time, drug therapy group and drug contrast group were given UDCA everyday until they were killed.After liver tissue was dyed with HE, the inflammatory and fibrotic degree of liver tissue and deposition change of reticular fiber were observed.The expression of PDGF-B and TIMP-1 was analyzed by immunohistochemistry.Compared with the normal contract group, the model group had shown typical hepatic fibrosis.The interval of reticular fiber had been formed extensively.Collagen in lobules of liver and hepatic sinusoid had grown up and deposited obviously.The number of reticular fiber in normal contrast group were 7.20±1.21, while those in model group 20.68±2.17 (P<0.01) .The expression of PDGF-B as well as the expression of TIMP-1 increased obviously.Drug therapy group could alleviate fibrotic severity in liver (P<0.01) and restrain the expression of PDGF-B and TIMP-1 (P<0.01) .It showed that UDCA could restrain reticular fiber and synthesis of PDGF-B and TIMP-1 so as to prevent from hepatic fibrosis in rat liver.