TGFβ1/Smads信号转导通路的变化在原发性肝癌发病机制中的作用

To explore the effect of alteration of TGFβ Ⅰ/Smads signaling pathway on pathogenesis of HCC.The expression in protein level and mRNA level of TGFβ Ⅰ, TGFβⅡ, Smad2, Smad4 and Smad7 in HCC liver tissues were detected by western blotting asssays and RT-PCR assays, and compared with normal liver control tissue and adjacent nonneoplastic tissues.The expression of TGFβⅡ and Smad4 mRNA in HCC liver tissues were significantly lower than that in normal control liver and adjacent nonneoplastic tissues.The expression of Smad7 mRNA and the ratio of Smad7/Smad4 in HCC liver were significantly higher than that in normal control liver and adjacent nonneoplastic liver tissues.The tendency of protein expression of TGFβⅡ, Smad4 and Smad7 were similar to mRNA expression in HCC liver tissues.If the TGFβⅠ/Smads signaling pathway was repressed, the information of growth inhibition could not be transmitted.It might be one of the factors of HCC pathogenesis.