血清cccDNA与HBVDNA、YMDD变异及肝炎复发的临床关系

Objective To investigate clinical relationship between cccDNA, Hepatitis B replication, YMDD mutants in sera and the hepatocyte injury of the liver.Methods PCR-molecular-beacon technique was used to examed cccDNA, HBV DNA and YMDD motif mutation in sera of subjects with HBV asymptomatic carriers (ASC) , chronic hepatitis (CHB) , liver cirrhosis (LC) and hepatocellular carcinoma (HCC) .Results 123 cccDNA positive samples were tested form all of 283 subjects sera (43.46%) , cccDNA was correlated with HBVDNA (x2=28.27, P<0.01) and with alanine aminotransferase (x2=48.46, P<0.01) .60 were examed ALT, HBVDNA, YMDD and cccDNA after treated with Lamivudine orally half a year, ALT were 32 abnormolity, HBVDNA were occurred in 24 subjects, including YMDD mutations occurred 18 and cccDNA 16 (correlated with cccDNA x2=48.46, P<0.01, x2=28.27, P<0.01 and P=0.046 repectivley) .Conclusion The occurrence of cccDNA in sera, may indicate HBV replication and liver damage.YMDD and cccDNA may be factors upon which the HBV replication status, the hepatic inflammation severity in anti-viral treatment.