The Precore Mutation in 52 Patients with Chronic Hepatitis B
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摘要: 本文采用多聚酶链反应 -单链构象多态分析 (PCR -SSCP)银染技术检测了 5 2例HBVDNA阳性慢性乙型肝炎患者的HBV前C区基因突变。结果有前C区突变株感染 32例 ,突变株检出率为 6 1 5 % ;突变株的检出以慢性重症肝炎最高 ,其次是慢性乙肝重度、中度患者 ,慢性乙肝轻度患者最低 ;抗 -HBe阳性患者前C区突变株检出率高于HBeAg阳性患者。表明HBV前C区突变与病情轻重及e系统血清标志有关。
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关键词:
- 乙型肝炎病毒 /
- 基因突变 /
- 多聚酶链反应-单链构象多态分析
Abstract: WT5”BZ] Single strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) and silver-stained technique was employed to detect HBV precore mutation in 52 cases with HBV DNA positive. 32 cases had precore mutation, the rate was 61.5%; the mutation rate in chronic server hepatitis was the highest (71.4%) . The mutation rate in anti-HBe positive cases was higher than in HBeAg cases, as a result the mutation rate in the two groups was higher than the compared groups. [WT5”HZ]-
Key words:
- Hepatitis B virus Gene Mutation PCR-SSCP /
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[1]杨玲 , 盛国光 , 李翰民 , 等 .慢性乙型肝炎HBV前C区基因突变与中医虚实证的关系。中西医结合肝病杂志 , 1998, 8 ( 3 ) ∶13 1 [][2 ]LodA , HdziyannisS , WellerI , etal.ContributionoflowleverHBVreplicationtocongtinuinginflammatoryactivityinpatientswithanti-HBepositivechronichepatitisBvirusinfection .Gut, 1984, 2 5∶12 83 [][3 ]OkamotoH , YosumotoY , AkahaneT , etal.Hepatitisviruswithprecoredefectsprevailinpersistentlyinfectedhostsalongwithserocon versiontoanti-HBe .JVirus, 1990 , 64∶12 98 [4]CarmanWF .PrecoresequencevariationinChineseisolatesofhep atitisBviruspathogenicity ?InfectDis, 1992 , 10 5∶12 7 [][5 ]AlbertiA .Dosinglenucleotidemutationresultinclinicallysignifi cantchangesinhepatitisBviruspathologenicity ?Hepatol, 1990 , 10∶2 68
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