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改良PCR法检测外周血单个核细胞中的HBV DNA与HBVcccDNA

陈瑞琳 蔺淑梅 张树林 叶峰 张曦 赵英仁

引用本文:
Citation:

改良PCR法检测外周血单个核细胞中的HBV DNA与HBVcccDNA

基金项目: 

陕西省自然科学基金(2003C244); 

详细信息
  • 中图分类号: R512.62;R450

Detection of the replicative state of HBV in peripheral blood mononuclear cells by improving PCR

Research funding: 

 

  • 摘要: 目的检测慢性HBV感染者外周血单个核细胞(PBMCs)中HBV DNA的存在状况。方法采用改良PCR法检测慢性HBV感染者PBMCs中HBV DNA、共价闭合环状DNA(cccDNA)。结果120例慢性HBV感染者PBMCs中HBV DNA、cccDNA阳性检出率分别为62.50%、45.83%,HBV DNA阳性检出率高于cccDNA(P<0.01);其中HBeAg阳性组PBMCs中HBV DNA(80.00%)和cccDNA阳性检出率(45.00%)分别明显高于HBeAg阴性组(61.67%)(30.00%)(P均<0.01);PBMCs中HBVDNA检测阳性者血清HBVDNA水平明显高于HBVDNA检测阴性者(P<0.01)。结论改良PCR法检测表明HBV DNA不仅可感染PBMCs,且部分参与复制;PBMCs中HBVDNA存在和复制能力与HBeAg阳性相关;PBMCs中HBV DNA阳性检出率与血清HBV DNA水平有一致性。

     

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  • 出版日期:  2008-05-20
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