The therapeutic efficacy of magnesium isoglycyrrhizinate on rats with non alcoholic steatohepatitis
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摘要: 目的探讨异甘草酸镁对非酒精性脂肪性肝炎(NASH)的治疗作用及其作用机制。方法雄性SD大鼠50只随机分为5组:正常组(N组)普通饲料喂养;模型Ⅰ组(M1组)和模型Ⅱ组(M2组)高脂饲料喂养;异甘草酸镁治疗组(T1组)和饮食治疗组(T2组)在喂饲高脂饲料12周后改为普通饲料喂养,其中T2组同时给予异甘草酸镁120 mg.kg-1.d-1尾静脉注射。12周末处死N和M1组大鼠;16周末处死M2、T1和T2组大鼠。观察各组大鼠肝脏组织病理学改变,并测定各组大鼠肝功能(血清AST、ALT)、脂代谢(血清TC、TG、HDL、LDL-C)及脂质过氧化(肝匀浆MDA、SOD、GSH)指标。结果 12周末M1组大鼠出现NASH,肝脏的脂肪变性程度和炎症活动度均显著增高,TC、TG、LDL-C、MDA明显升高,HDL、SOD和GSH明显降低(P<0.05);16周末,M2组大鼠NASH程度进一步加重,并出现AST和ALT的明显升高(P<0.05)。和M1、M2组相比,T1组脂肪变性和炎症活动程度明显减轻,肝功能、脂代谢和脂质过氧化各项指标均改善(P<0.05);而T2组差异无统计学意义...Abstract: Objective To explore the therapeutic efficacy and the mechanism of magnesium isoglycyrrhizinate on rats with non acoholic steatohepatitis.Methods A total of 50 male Srague Dawley rats were randomly assigned into 5 groups.The rats in the control(N) group were fed with common food,the model I(M1) group and model II(M2) groups were fed with high-fat diets,the treatment group(T1) rats were treated with magnesium isoglycyrrhizinate and dietary therapy group(T2) rats were fed with high-fat diets until 12 weeks.The T2 group rats were then treated with common food and the rats in T1 group were treated with magnesium isoglycyrrhizinate(120mg·kg-1·d-1).The rats in N and M1 group were killed at the end of the12th week and while the ones in M2,T1 and T2 group at the end of the 16th week.Liver function tests(AST and ALT),lipid metabolism(TC,TG,HDL and LDL-C) and lipid peroxidation(MDA,SOD and GSH) parameters were measured and histopathological changes of liver were observed.Results The rats in M1 group developed NASH after 12 weeks.The rats in the N group had aggravated hepatic fatty degeneration and inflammation,increased TC,TG,LDL-C and MDA,and decreased HDL,SOD and GSH compared to the control group(P<0.05).The degree of NASH aggravated,and the AST and ALT levels were increased after week 16 in the M2 group(P<0.05).The parameters and the degree of severity of hepatic fat degeneration in T1 group were all improved compared to the M1 and M2 groups(P<0.05).But there was no significant change in the T1 group(P>0.05).Conclusion Magnesium isoglycyrrhizinate therapy can improve lipid metabolism disorder,anti-lipid peroxidation,and anti-inflammation in patients with non acoholic steatohepatitis.
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Key words:
- fatty liver /
- magnesium isoglycyrrhizinate
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[1]裴强,王晓素,王宪波,等.非酒精性脂肪性肝炎发病机制的研究进展[J].临床肝胆病杂志,2008,24(4):304-306. [2]邵桂霞,张献共,黄光平,等.深圳地区28384例体检者体重指数与脂肪肝分布的探讨[J].中华肝脏病杂志,2003,11(6):372-373. [3]王慧超,叶红军,韦始亮,等.复方甘草甜素脂质体干预大鼠非酒精性脂肪性肝炎的疗效观察[J].临床肝胆病杂志,2008,24(3):199-201. [4]王泰龄,刘霞,周元平,等.慢性肝炎炎症活动度及纤维化程度计分方案[J].中华肝脏病杂志,1998,6(4):195-197. [5]Musso G,Gambino R,Cassader M,et al.A meta-analysis of randomized trials for the treatment of nonalcoholic fatty liver disease[J].Hepatology,2010,52(1):79-104. [6]Garvin JL,Ortiz PA.The role of reactive oxygen species in the regulation of tubular function[J].Acta Physiol Scand,2003,179(3):225-232. [7]Koruk M,Taysi B,Savas MC,et al.Oxidative stress and enzymatic antioxidant status in patients with nonalcoholic steatohepatitis[J].Ann Clin Lab Sci,2004,34(1):57-62. [8] 茅益民,李军,隋云华,等.异甘草酸镁治疗ALT升高的慢性肝病的多中心、随机、双盲、多剂量、阳性药物平行对照临床研究[J].中华肝脏病杂志,2009,17(11):847-851.
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