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丙型肝炎核心抗原检测在丙型肝炎诊断中的意义

杨杰 崔敬 刘春 魏枫华 窦晓光

引用本文:
Citation:

丙型肝炎核心抗原检测在丙型肝炎诊断中的意义

基金项目: 

国家“十一五”传染病重大专项课题(20082X10206); 辽宁省科学技术计划重大重点项目(2009225010-7); 

详细信息
  • 中图分类号: R512.63

Detection of HCV core antigen and its diagnostic significance 

Research funding: 

 

  • 摘要:

    目的了解丙型肝炎核心抗原(HCV-cAg)检测方法的敏感性及特异性,确定具有临床意义的S/CO值,探讨其在丙型肝炎诊断中的意义。方法使用ELASA方法检测丙型肝炎核心抗原,RT-PCR检测HCV RNA定量,观察不同S/CO值所对应的HCV RNA定量之间的关系,以HCV RNA为诊断金标准,列四格表做诊断实验。结果 HCV-cAg抗原检测的敏感性为87.05%,特异性为76.67%,阳性预测值为96.53%,阴性预测值为44.23%。结论 (1)随着HCV-cAg的S/CO值逐渐增大,其与HCV RNA阳性符合率明显增高,随着HCV-cAg的S/CO值减小,其与HCV RNA阴性符合率明显增高;(2)S/CO值=2可以作为临床判断HCV感染病毒血症存在的一个标准;(3)本实验的敏感性和特异性较好,检测方法简单,可以作为丙型肝炎临床诊断的补充试验及筛查。

     

  • [1]Lavanchy D.Evoling Epidemiology of hepatitis C virus[J].Clin Microbiol Infect, 2011, 17 (2) :107-115.
    [2]刘丽君, 魏来.丙型肝炎病毒的流行病学[J].传染病信息, 2007, 20 (5) :261-264.
    [3]丁进, 岳向荣, 张红梅.302例老年患者丙型肝炎检测分析[J].检验医学与临床, 2009, 6 (17) :1474-1475.
    [4]Kesli R, Polat H, Terzi Y, et al.Comparison of a newly de-veloped automated and quantitative hepatitis C virus (HCV) core antigen test with the HCV RNA assay for clinical useful-ness in confirming anti-HCV results[J].J Clin Microbiol, 2011, 49 (12) :4082-4093.
    [5]Hosseini-Moghaddam SM, Iran-Pour E, Rotstein C, et al.Hepatitis C core Ag an dis clinic-al applicability:potentialadvantages and disadvantages for diagnosis for diagnosis andfoll-ow-up?[J].Rev Med Virol, 2012, 22 (3) :156-165.
    [6]Yuksel P, Caliskan R, Ergin S, et al.Diagnostic value of de-termination of hepatitis C virus core antigen[J].TransfusionApheresis Sci, 2011, 45 (3) :247-250.
    [7]Cao H, Zhang K, Shu X, et al.Detection of core antigen ofhepatitis virus C in patients infected with hepatitis C and B[J].Chin J Hepatol, 2011, 19 (10) :726-728. (in Chi-nese) 曹红, 张卡, 舒欣, 等.合并乙型肝炎病毒感染对丙型肝炎病毒核心抗原检测的影响[J].中华肝脏病杂志, 2011, 19 (10) :726-728.
    [8]Mori Y, Morishi K, Matsuura Y.Hepatitis C virus core pro-tein:its coordinate roles with PA 28γin metabolic abnormali-ty and carcinogenicity in the liver[J].Int J Biochem Cell Bi-ol, 2008, 40 (8) :1437-1442.
    [9]Seto WK, Lai CL, Fung J, et al.Natural history of chronichepatitis C:genotype 1 versus genotype 6[J].J Hepatol, 2010, 53 (3) :444-448.
    [10]Baranov AV, Maleev W.Association between HCV RNA lev-el and anti-HCV antibodies During chronic hepatitis C[J].Zh Mikrobiol Epidemiol immunobiol, 2009, (5) :19-22.
    [11]Busch MP, Glynn SA, Stramer SL, et al.NHLBI-REDSNAT Study Group (2005) .A new strategy for estimatingrisks of transfusion-transmitted viral infections based onrates of detection of recently infected donors[J].Transfu-sion, 2005, 45 (2) :254-264.
    [12]Chevaliez S.Virological tools to diagnose and monitor hepatitisC infection[J].Clinic Microbiol Infect, 2011, 17 (2) :116-121.
    [13]Morota K, Fujinami R, Kiunkawa H, et al.A new sensitiveand automated chemiluminescent microparticle immunoassayfor quantitative determination of hepatitis C virus core anti-gen.[J].Virol Methods, 2009, 157 (1) :8-14.
    [14]Nübling CM, Unger G, Chudy M, et al.Sensitivity of HCVcore antigen And HCV-RNA detection in the early infectionphase[J].Transfusion, 2002, 48 (8) :1037.
    [15]Widell A, wolnegren V, Pieksma F, et al.Detection of hepa-titis C core antigen in the serolo-gical window-phase[J].Transfus Med, 2002, 12 (2) :107.
    [16]Wang GH, Zhang HQ, Li SB, et al.Preparation and testingof hepatitis C core antigen ELISA reagent[J].Chin J BloodTransfusion, 2004, 17 (5) :299-300. (in Chinese) 王国华, 张贺秋, 李少波, 等.丙型肝炎病毒核心抗原检测试剂的研制及初步应用[J].中国输血杂志, 2004, 17 (5) :299-300.
    [17]Meng SF, Li XH, Yin HZ, et al.Establishment of detectiontest for hepatitis C virus antigen[J].Chin J Exp Clin Virol, 2001, 15 (3) :287-290. (in Chinese) 孟淑芳, 李秀华, 尹红章, 等.丙型肝炎病毒抗原检测方法的建立[J].中华实验和临床病毒学杂志, 2001, 15 (3) :287-290.
    [18]Ergunay K, Sener B, Alp A, et al.Utility of a commercialquantitative hepatitis C virus core antigen assay in a diagnos-tic laboratory setting[J].Diaqn Microbiology Infect Dis, 2011, 70 (4) :486-491.
    [19]Gu S, Liu J, Zhang H, et al.Core antigen tests for hepatitisC virus:a meta-analysis[J].Mol biol Rep, 2012, 39 (8) :81-123.
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