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急性酒精性脂肪肝斑马鱼模型的建立

戴文聪 刘莉 王坤元 侯金林

引用本文:
Citation:

急性酒精性脂肪肝斑马鱼模型的建立

详细信息
  • 中图分类号: R575.5

Establishment of a zebrafish model of alcohol-induced acute hepatic steatosis

  • 摘要:

    目的建立一种简单易行、重复性好的急性酒精性脂肪肝斑马鱼模型。方法选取野生型斑马鱼288条,随机均分为对照组和2%、2.5%、3%乙醇处理组,于胚胎受精后5 d给予相应体积浓度比乙醇处理。观察斑马鱼一般生存率和表型变化,用整体油红染色评判脂肪肝发生率,石蜡切片观察肝组织病理形态。结果 2%、2.5%和3%乙醇处理组斑马鱼32 h内生存率分别为100%、(91.21±1.61)%、0%。3%乙醇处理24 h即出现畸形。整体油红染色显示2%乙醇处理组斑马鱼在处理32 h后脂肪肝发生率显著高于对照组(P=0.002)。病理切片可见2%乙醇处理32 h后斑马鱼肝细胞肿胀,肝内脂滴沉积增多,脂肪肝形成。结论利用2%乙醇处理胚胎受精后5 d的幼鱼32 h成功地建立急性酒精性脂肪肝斑马鱼模型,为进一步开展急性酒精性脂肪肝的发病机制研究和药物筛选奠定实验基础。

     

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  • 出版日期:  2013-04-20
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