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慢性HBV感染者肝脏HBV cccDNA含量相关因素分析

陈嵬 吴峰 窦晓光 张琳

引用本文:
Citation:

慢性HBV感染者肝脏HBV cccDNA含量相关因素分析

基金项目: 

国家“十一五”传染病重大专项课题(2008ZX10002-004); 国家自然科学基金项目(30972612); 

详细信息
  • 中图分类号: R512.62

Analysis of related factors for intrahepatic HBV cccDNA level in patients with chronic HBV infection

Research funding: 

 

  • 摘要:

    目的探讨慢性HBV感染者肝组织中HBV cccDNA含量与血清病毒标志物、HBV DNA及肝脏病理分级的关系,为临床评价抗病毒治疗效果及疗程确定提供理论依据。方法以2007年5月-2008年2月住院的30例慢性HBV感染者为研究对象,应用实时荧光定量聚合酶链反应(RT-PCR)方法检测患者肝组织中HBV cccDNA、肝组织总HBV DNA(HBV tDNA)和血清HBVDNA,同时用化学发光免疫分析法检测HBsAg、HBeAg定量,分析感染者肝组织内HBV cccDNA与肝组织内HBV DNA、血清HBVDNA、HBsAg及HBeAg定量水平之间的关系,并比较肝组织中HBV cccDNA含量与肝脏病理炎症和纤维化分级的关系。采用Pear-son简单相关和Spearman等级相关法进行相关性分析。结果 30例慢性HBV感染者肝组织中均可检出HBV cccDNA,范围在3.15×103~1.06×107拷贝/mg;肝组织cccDNA定量与肝组织总HBV DNA定量呈正相关(r=0.375,P<0.05),与血清hbv r="0.174,P">0.05);肝组织中H...

     

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  • 出版日期:  2013-06-20
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