Analysis of serum autoantibodies in patients with HBV infection
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摘要:
目的探讨HBV感染者体内自身免疫的变化情况。方法选取120例HBV感染者作为HBV阳性组,其中HBV携带者,乙型肝炎,肝硬化,肝癌患者各30例;同时选取50例健康体检者作为对照组。采用间接荧光法测120例HBV感染者及50例健康体检者血清中抗核抗体(ANA)及抗平滑肌抗体(SMA),采用酶联免疫吸附法测抗线粒体抗体M2型(AMA-M2),采用散色比浊法测类风湿因子(RF),采用印迹法测抗可溶性肝抗原/抗肝胰抗原抗体(SLA/LP)、抗肝肾微粒体抗体I型(LKM-1)、抗肝细胞浆抗体I型(LC-1)。结果 (1)HBV阳性组自身抗体总阳性率为48.3%,高于正常对照组的4%(χ2=30.375,P<0.05);(2)HBV携带组、肝炎组、肝硬化组、肝癌组自身抗体阳性率分别为40%、40%、56.7%、56.7%,组间比较χ2=3.337,P=0.343,差异无统计学意义;(3)自身抗体以ANA、RF为主,ANA滴度以1∶100为主,荧光类型以"均质型"、"颗粒型"为主;(4)对ANA滴度≥1∶320者进行肝谱分析,肝硬化组有2例AMA-M2阳性,说明HBV感染有自身免疫肝病发生的可...
Abstract:ObjectiveTo investigate the condition of autoimmunity in patients infected with the hepatitis B virus (HBV). MethodsSerum samples were collected from 120 HBV-infected patients and 50 healthy controls. Antinuclear antibodies (ANA) and smooth muscle antibodies (SMA) were detected by indirect immunofluorescence. Antimitochondrial antibody-M2 antigen (AMA-M2) was detected by enzyme-linked immunosorbent assay. Rheumatoid factor (RF) was detected by a scatter turbidity method. Antibodies against soluble liver antigen (SLA/LP), liver kidney microsomal (LKM-1), and liver cystosol antigen type 1 (LC-1) were detected by immunoblotting. ResultsThe positive rate autoantibodies was significantly higher in the HBV-infected patients (48.3% vs. controls: 4.0%, χ2=30.375, P<0.05. However, among the HBV-infected patients, there were no significant differences in the positive rates of autoantibodies between the sub-groups of HBV carriers (40.0%), hepatitis (40.0%), hepatic sclerosis (56.7%), and hepatic cancer (56.7%) (χ2=3.337, P=0.343). ANA and RF were the most frequently detected autoantibodies. The ANA titer was mostly 1∶100, and the ANA pattern was mostly of the “homogeneous type” or “granular type”. The liver profile (IgG) was taken for patients with ANA titer ≥1∶320 and as a result there were only two positive samples of AMA-M2 in the hepatic sclerosis group, indicating that HBV infection can induce autoimmune liver disease. The levels of aspartate aminotransferase were significantly different between the HBV-infected patients who were negative for autoantibodies and those who were positive for autoantibodies (P=0.007), indicating that the autoantibodies contributed to liver function damage. ConclusionHBV-infected individuals express many kinds of autoantibodies, which may promote damage to liver function and should be paid attention to by treating physicians.
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