异甘草酸镁对D-氨基半乳糖致急性肝衰竭大鼠模型的保护作用

李小鹏; 李明; 雷弯; 张伦理

doi:10.3969/j.issn.1001-5256.2016.03.032

异甘草酸镁对D-氨基半乳糖致急性肝衰竭大鼠模型的保护作用

DOI: 10.3969/j.issn.1001-5256.2016.03.032

南昌大学第一附属医院感染病科

基金项目:

中国肝炎防治基金会-天晴肝病研究基金课题(20120055)；江西省卫生厅中医药课题(1210452992)；

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中图分类号: R575.3
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出版历程
- 出版日期: 2016-03-20

Protective effect of magnesium isoglycyrrhizinate in rats with acute liver failure induced by D- galactosamine

Department of Infectious Diseases, The First Affiliated Hospital of Nanchang University

Research funding:

摘要

摘要:
目的探讨异甘草酸镁对D-氨基半乳糖致急性肝衰竭（ALF）大鼠模型的保护作用及其机制。方法 90只SD大鼠随机分为正常组（A组,n=10）、模型组（B组,n=20）、异甘草酸镁低剂量组（C组,n=20）、中剂量组（D组,n=20）和高剂量组（E组,n=20）。A组腹腔内仅注射等量无菌生理盐水,其余4组均一次性腹腔注射10%D-氨基半乳糖。C、D、E 3组在造模30 min后均于尾静脉注射异甘草酸镁注射液（25、50和100 mg/kg）。建模24和48 h后分别取肝组织行HE染色观察肝组织结构的病理变化,收集血清检测各组大鼠肝功能指标[ALT、AST、TBil、白蛋白（Alb）]、促炎症因子[肿瘤坏死因子（TNF）α、白细胞介素（IL）1β、干扰素（IFN）γ]和抑炎症因子[IL-4、IL-10、转化生长因子（TGF）β]水平。计量资料多组间比较采用单因素方差分析,进一步两两比较采用SNK-q检验。结果 5组大鼠的血清ALT、AST、TBil和Alb水平比较差异均有统计学意义（F值分别为74.6、108.9、25.1、58.4,P值均<0.05）;与A组比较,B组血清ALT、AST...
- 肝功能衰竭,急性 /
- 异甘草酸镁 /
- 细胞因子类 /
- 大鼠,Sprague-Dawley
Abstract:
Objective To investigate the protective effect of magnesium isoglycyrrhizinate( Mg IG) in rats with acute liver failure induced by D- galactosamine( D- Gal N) and related mechanisms. Methods A total of 90 Sprague- Dawley rats were randomly divided into normal group( group A,n = 10),model group( group B,n = 20),low- dose Mg IG group( group C,n = 20),medium- dose Mg IG group( group D,n = 20),and high- dose Mg IG group( group E,n = 20). The rats in group A received intraperitoneal injection of the same volume of normal saline,and those in the other groups received single intraperitoneal injection of 10% D- Gal N. At 30 minutes after establishment of the model,the rats in groups C,D,and E received tail vein injection of Mg IG injection( 25,50,and 100 mg / kg). At 24 and 48 hours after establishment of the model,HE staining was performed to observe the pathological changes in the liver,and serum was collected to measure the levels of liver function parameters [alanine aminotransferase( ALT),aspartate aminotransferase( AST),total bilirubin( TBil),and albumin( Alb) ],proinflammatory factors [tumor necrosis factor- α( TNFα),interleukin- 1β( IL- 1β),and interferon-γ( IFNγ) ],and anti- inflammatory factors [interleukin 4( IL- 4),interleukin- 10( IL- 10),and transforming growth factor β( TGFβ) ]in rats. The one- way analysis of variance was applied for comparison between multiple groups,and the Student- Newman-Keuls test was applied for comparison between any two groups. Results The serum levels of ALT,AST,TBil,and Alb showed significant differences across the five groups( F = 74. 6,108. 9,25. 1,and 58. 4,all P < 0. 05); compared with group A,group B had significant increases in the serum levels of ALT,AST,and TBil and a significant reduction in the serum level of Alb( all P < 0. 05); compared with group B,groups C,D,and E had significant reductions in the serum levels of ALT,AST,and TBil and a significant increase in the serum level of Alb,and these parameters showed the most significant changes in group E( all P < 0. 05). The serum levels of TNFα,IL- lβ,IFNγ,IL-4,IL- 10,and TGFβ showed significant differences across the five groups( F = 75. 1,58. 9,25. 4,43. 6,66. 4,and 86. 8,all P <0. 05); compared with group A,group B had significant increases in the serum levels of TNFα,IL- lβ,IFNγ,IL- 4,IL- 10,and TGFβ( all P < 0. 05); compared with group B,groups C,D,and E had significant reductions in the serum levels of TNFα,IL- lβ,and IFNγand significant increases in the serum levels of IL- 4,IL- 10,and TGFβ,and these parameters showed the most significant changes in group E( all P < 0. 05). Conclusion Mg IG can reduce inflammatory response through reducing proinflammatory factors and increasing anti- inflammatory factors,and thus exert protective effect on rats with ALF.
- liver failure,acute /
- magnesium isoglycyrrhizina /
- cytokines /
- rats,sprague-Dawley

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参考文献(11)

[1]ZHU LY.The Progress of magnesuim isoglycyrrhizinate in clinical research[J].J Hainan Med Coll,2013,19(12):1776-1780.(in Chinese)朱礼尧.浅述异甘草酸镁临床研究进展[J].海南医学院学报,2013,19(12):1776-1780.

[2]QIAO Y,HAO RL,TANG ZQ.Clinical study on telbivudine and magnesium isoglycyrrhizinate in treatment of severe hepatitis B[J/CD].Chin J Exp Clin Infect Dis:Electronic Edition,2015,9(5):670-673.(in Chinese)乔英,郝润来,唐中权.替比夫定和异甘草酸镁治疗重型乙型肝炎的临床研究[J/CD].中华实验和临床感染病杂志:电子版,2015,9(5):670-673.

[3]GU X,DONG LP,LIU CY,et al.Protective effect of magnesium isogiycyrrhizinate on acute hepatic injury induced by CCl4 in Mice[J].China Pharmacist,2009,12(1):37-39.(in Chinese)顾新,董丽萍,刘宇宸,等.异甘草酸镁对急性四氯化碳肝损伤小鼠保护作用的研究[J].中国药师,2009,12(1):37-39.

[4]XIE YF,LIU JG,LIU Q,et al.Protective effect of magnesium isogiycyrrhizinate on acute alcoholic liver injury in mice[J].J Pract Med,2010,26(3):381-383.(in Chinese)谢议凤,刘建国,刘庆,等.异甘草酸镁对小鼠急性酒精性肝损伤的保护作用[J].实用医学杂志,2010,26(3):381-383.

[5]JING XZ,LIU J,QIAN H.The therapeutic efficacy of magnesium isoglycyrrhizinate on rats with non alcoholic steatohepatitis[J].J Clin Hepatol,2011,27(3):295-297.(in Chinese)经小珍,刘竞,钱海.异甘草酸镁治疗大鼠非酒精性脂肪性肝炎及其作用机制[J].临床肝胆病杂志,2011,27(3):295-297.

[6]ZHAO J,LIU HH,TAN BY.Experimental study of magnesium isog Iycyrrhizinate treatment on liver injury in severe a cute pancreatitis[J].J Med Res,2014,43(11):147-149.(in Chinese)赵建,刘红辉,谭波宇.异甘草酸镁对重症急性胰腺炎肝损伤治疗作用的实验研究[J].医学研究杂志,2014,43(11):147-149.

[7]HUANG JJ,LI XP,CHEN XY,et al.Expression of intestinal defensin-5,soluble phospholipase A2 and lysozyme and the relation to bacterial translocation in rat models of acute liver failure[J].Chin J Hepatol,2014,22(12):138-143.(in Chinese)黄娟君,李小鹏,程晓宇,等.急性肝衰竭大鼠模型肠道防御素-5、分泌型磷脂酶A2和溶菌酶表达变化及与细菌移位的关系[J].中华肝脏病杂志,2014,22(12):138-143.

[8] Chinese Society of Hepatology,Chinese Society of Infectious Diseases,Chinese Medical Association.The guideline of prevention and treatment for chronic hepatitis B(2010 version)[J].J Clin Hepatol,2011,27(1):Ⅰ-ⅩⅥ.(in Chinese)中华医学会肝病分会,感染病学分会.慢性乙型肝炎防治指南(2010年版)[J].临床肝胆病杂志,2011,27(1):Ⅰ-ⅩⅥ.

[9] Liver Failure and Artificial Liver Group,Chinese Society of Infectious Diseases,Severe Liver Disease and Artificial Liver Group,Chinese Society of Hepatology.Guideline for diagnosis and treatment of liver failure(2012 Version)[J].Chin J Clin Infect Dis,2012,5(6):321-330.(in Chinese)中华医学会感染病学分会肝衰竭与人工肝学组,肝病分会重型肝炎与人工肝学组.肝衰竭诊疗指南(2012版)[J].中华临床感染病杂志,2012,5(6):321-330.

[10]LUCIA AP,CHARALAMBOS GA,QUENTIN MA,et al.Role of monocytes and macrophages in experimental and human acute liver failure[J].World J Gastroenterol,2010,16(15):1811-1819.

[11]CHEN N,ZHANG LL,XUE GR,et al.An initial study on the imbalance of pro-inflammatory cytokine and anti-inflammatory cytokine in patients with acute liver failure induced by HBV[J].Guangdong Med J.2012,33(8):1150-1152.(in Chinese)程娜,张伦理,徐国荣,等.HBV感染的急性肝衰竭患者促炎因子及抗炎因子失衡的初步研究[J].广东医学,2012,33(8):1150-1152.

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