中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

成纤维细胞生长因子21对小鼠肝纤维化的作用及其机制

王大秀 韩继武

引用本文:
Citation:

成纤维细胞生长因子21对小鼠肝纤维化的作用及其机制

DOI: 10.3969/j.issn.1001-5256.2018.06.017
详细信息
  • 中图分类号: R575.2

Effect of fibroblast growth factor 21 on hepatic fibrosis in mice and its mechanism

  • 摘要: 目的探索成纤维细胞生长因子(FGF)21对小鼠肝纤维化的作用及机制。方法将40只雄性ICR小鼠随机分为对照组(n=10)、模型组(CCl4处理)(n=15)、治疗组(CCl4+1.0 mg/kg FGF21处理)(n=15)。连续处理36 d后取血清及肝组织。检测ALT、AST、ALP、TBil、IL-6、IL-1β、TNFα水平;Masson染色观察病理改变;4-羟脯氨酸(4-Hyp)试剂盒检测肝内4-Hyp水平;real-time PCR检测肝胶原蛋白Ⅰ(CollagenⅠ)、α-平滑肌肌动蛋白(α-SMA)、TGFβ、IL-6、IL-1β、TNFαmRNA水平。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果 Masson染色结果显示,模型组肝纤维化程度较对照组明显加重,治疗组肝纤维化程度较模型组明显减轻;生化检测结果显示,模型组小鼠血清ALT、AST、ALP、TBil水平明显高于对照组,差异均有统计学意义(P值均<0.05);治疗组小鼠血清ALT、AST、ALP、TBil水平较模型组明显下降,差异均有统计学意义(P值均<0.05)。血清E...

     

  • [1]BATALLER R, BRENNER DA.Liver fibrosis[J].J Clin Invest, 2005, 115 (2) :209-218.
    [2]TSUKADA S, PARSONS CJ, RIPPE RA.Mechanisms of liver fibrosis[J].Clin Chim Acta, 2006, 364 (1-2) :33-60.
    [3]FRIEDMAN SL.Stellate cells:A moving target in hepatic fibrogenesis[J].Hepatology, 2004, 40 (5) :1041-1043.
    [4]WALLACE MC, FRIEDMAN SL, MANN DA.Emerging and disease-specific mechanisms of hepatic stellate cell activation[J].Semin Liver Dis, 2015, 35 (2) :107-118.
    [5]NAGAKI M, MORIWAKI H.Implication of cytokines:Roles of tumor necrosis factor-alpha in liver injury[J].Hepatol Res, 2008, 38 (Suppl 1) :s19-s28.
    [6]BERASAIN C, CASTILLO J, PERUGORRIA MJ, et al.Inflammation and liver cancer:New molecular links[J].Ann N Y Acid Sci, 2009, 1155 (1) :206-221.
    [7]ZHANG W, JIA JD.Pathogenesis and new therapeutic targets of liver fibrosis[J].J Clin Hepatol, 2017, 33 (3) :409-412. (in Chinese) 张伟, 贾继东.肝纤维化的发病机制及治疗新靶点[J].临床肝胆病杂志, 2017, 33 (3) :409-412.
    [8]PENG Q, ZHAO SJ, LI W.Research advances in signaling pathways involved in the development of hepatic fibrosis[J].J Clin Hepatol, 2017, 33 (5) :954-958. (in Chinese) 彭琴, 赵绅君, 李武.肝纤维化相关信号转导通路研究进展[J].临床肝胆病杂志, 2017, 33 (5) :954-958.
    [9]MARKAN KR, NABER MC, AMEKA MK, et al.Circulating FGF21 is liver derived and enhances glucose uptake during refeeding and overfeeding[J].Diabetes, 2014, 63 (12) :4057-4063.
    [10]DEGIROLAMO C, SABBA C, MOSCHETTA A.Therapeutic potential of the endocrine fibroblast growth factors FGF19, FGF21 and FGF23[J].Nat Rev Drug Discov, 2016, 15 (1) :51-69.
    [11]YU Y, BAI F, LIU Y, et al.Fibroblast growth factor (FGF21) protects mouse liver against D-galactose-induced oxidative stress and apoptosis via activating Nrf2 and PI3K/Akt pathways[J].Mol Cell Biochem, 2015, 403 (1-2) :287-299.
    [12]REN GP, JIANG YY, LIU MY, et al.Utilization of SUMO expression vector to efficiently express mouse FGF-21 and studying of its bioactivity[J].J Northeast Agric Univ, 2009, 40 (5) :62-67. (in Chinese) 任桂萍, 姜媛媛, 刘铭瑶, 等.SUMO融合系统高效表达可溶性鼠源FGF-21及其活性的研究[J].东北农业大学学报, 2009, 40 (5) :62-67.
    [13]XU P, YE X, ZHANG Y, et al.Long-acting hypoglycemic effects of PEGylated FGF21 and insulin glargine in mice with type 1 diabetes[J].J Diabetes Complicetions, 2015, 29 (1) :5-12.
    [14]XU J, LLOYD DJ, HALE C, et al.Fibroblast growth factor 21 reverses hepatic steatosis, increases energy expenditure, and improves insulin sensitivity in diet-induced obese mice[J].Diabetes, 2009, 58 (1) :250-259.
    [15]CAMPOREZ JP, JORNAYVAZ FR, LEE HY, et al.Cellular mechanism by which estradio protects female ovariectomized mice from high-fat diet-induced hepatic and muscle insulin resistence[J].Endocrinology, 2013, 154 (3) :1021-1028.
    [16]YE D, WANG Y, LI H, et al.FGF21 protects against acetaminophen-induced hepatotoxicity by potentiating PGC-1alpha-mediated antioxidant capacity in mice[J].Hepatology, 2014, 60 (3) :977-989.
    [17]XU P, ZHANG Y, WANG W, et al.Long-term administration of fibroblast growth factor 21 prevents chemically-induced hepatocarcinogenesis in mice[J].Dig Dis Sci, 2015, 60 (10) :3032-3043.
    [18]LIU Y, ZHAO C, XIAO J, et al.Fibroblast growth factor 21 deficiency exacerbates chronic alcohol-induced hepatic steatosis and injury[J].Sci Rep, 2016, 6:31026.
    [19]YANG C, LU W, LIN T, et al.Activation of liver FGF21 in hepatocarcinogenesis and during hepatic stress[J].BMC Gastroetero, 2013, 13:67.
    [20]DUSHAY, CHUI PC, GOPALAKRISHNAN GS, et al.Increased fibroblast growth factor 21 in obesity and nonalcoholic fatty liver disease[J].Gastroenterology, 2010, 139 (2) :456-463.
    [21]COSKUN T, BINA HA, SCHNEIDER MA, et al.Fibroblast growth factor 21 corrects obesity in mice[J].Endocrinnology, 2008, 149 (12) :6018-6027.
  • 加载中
计量
  • 文章访问数:  1960
  • HTML全文浏览量:  25
  • PDF下载量:  361
  • 被引次数: 0
出版历程
  • 收稿日期:  2017-11-13
  • 出版日期:  2018-06-20
  • 分享
  • 用微信扫码二维码

    分享至好友和朋友圈

目录

    /

    返回文章
    返回