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环境内分泌干扰物双酚A对非酒精性脂肪性肝病大鼠模型肠道黏膜屏障的影响

丁雯瑾 袁涛 沈峰 周达 孙超 范建高 陈源文

引用本文:
Citation:

环境内分泌干扰物双酚A对非酒精性脂肪性肝病大鼠模型肠道黏膜屏障的影响

DOI: 10.3969/j.issn.1001-5256.2018.06.027
基金项目: 

国家自然科学基金资助项目(81400610); 教育部留学回国人员科研启动基金项目(201404802); 上海市卫生和计划生育委员会青年科研项目(20134Y043); 上海交通大学医工交叉研究基金资助项目(YG2016MS72); 黎介寿院士肠道屏障研究专项基金(LJS_201214,LJS_201319); 

详细信息
  • 中图分类号: R-332;R575.5

Influence of bisphenol A on intestinal mucosal barrier in rats with nonalcoholic fatty liver disease

Research funding: 

 

  • 摘要:

    目的研究环境内分泌干扰物双酚A(BPA)对非酒精性脂肪性肝病(NAFLD)大鼠炎症反应的影响,以及对肠道黏膜屏障的损害作用。方法将18只成熟雄性SD大鼠随机分为3组:正常组、NAFLD组和NAFLD+BPA组,每组6只。光镜下观察肝脏病理改变,ELISA检测血清TNFα、IL-1β、IL-6和IL-8等炎症因子,鲎试剂终点比色法检测内毒素水平,免疫荧光法观察肠道黏膜Occludin蛋白的表达情况,及实时聚合酶链式反应测定肠道黏膜外周蛋白ZO-1 mRNA的改变。计量资料多组间比较采用单因素方差分析,进一步两两比较用SNK-q检验。结果肝脏病理学证实NAFLD模型建模成功。100 nmol/L BPA摄入8周后,TNFα、IL-6、IL-8炎症因子表达上调[分别为(127.65±22.40)pg/ml、(199.34±17.46)pg/ml和(258.79±12.82)pg/ml]伴内毒素水平增高[(0.88±0.26)EU/ml],与正常组[分别为(64.87±10.83)pg/ml、(91.27±9.82)pg/ml、(123.76±19.68)pg/ml和(0.27±0.09)EU...

     

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  • 收稿日期:  2017-12-19
  • 出版日期:  2018-06-20
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